Lapiński Tadeusz Wojciech, Pogorzelska Joanna, Grzeszczuk Anna, Swiderska Magdalena, Kowalczuk Oksana, Nikliński Jacek, Flisiak Robert
Przegl Epidemiol. 2014;68(1):11-5, 105-8.
CD40 receptor is activated by ligand CD40L (CD154) which is synthesized in inflammation by NK cells, monocytes and lymphocytes B. TRAF proteins are activated in cells by CD40 stimulation and next they stimulate different enzymatic pathways. High concentrations of CD40L stimulate CD40, and consequently STAT enzyme system inhibits the expression ofnonstructural proteins ofHCV NS3 and NS5A and E2 core in infected human hepatocytes. PURPOSE. The aim of the study was to evaluate the concentration of soluble components of the complex: sCD40 and sCD40L in the serum of patients infected with HCV and HCV/HIV-1 co-infected. The effect ofHCV genotype, HIV and HCV viral load and rs12979860 polymorphism on serum sCD40 and sCD40L was established among the patients. The influence of the number of CD3+, CD4+ and CD8+ on the concentrations of sCD40 and sCD40L was evaluated in the HIV-1 infected group MATERIALS AND METHODS. Serum concentrations of sCD40 and sCD40L were determined using ELISA in 68 HCV infected patients including 39 HCV monoinfected and 29 HCV/HIV-1 co-infected. RESULTS. Serum concentration of sCD40 and sCD40L was significantly higher in HCV and HCV/HIV coinfected patients compared to healthy subjects (25.7 and 23.2 v. 8.5 pg/ml and 12.7 and 7.3 v. 0.79 ng/ml). The concentration of sCD40L in patients with genotype CC rs12979860 was significantly higher compared to patients with Non-CC genotypes (11.8 v. 7.6 ng/ml, p < 0.018). CONCLUSIONS. High levels of sCD40 and sCD40L were detected among patients with chronic HCV and HCV/ HIV-1 infection The high concentration of sCD40L correlates with CC rs12979860 genotype.
CD40受体由配体CD40L（CD154）激活，CD40L由自然杀伤细胞、单核细胞和B淋巴细胞在炎症过程中合成。TRAF蛋白在细胞中被CD40刺激激活，随后刺激不同的酶促途径。高浓度的CD40L刺激CD40，因此STAT酶系统抑制受感染人类肝细胞中丙型肝炎病毒NS3和NS5A以及E2核心的非结构蛋白的表达。目的。本研究的目的是评估复合物的可溶性成分：丙型肝炎病毒感染患者以及丙型肝炎病毒/人类免疫缺陷病毒-1（HCV/HIV-1）合并感染患者血清中的可溶性CD40（sCD40）和可溶性CD40L（sCD40L）的浓度。在患者中确定了丙型肝炎病毒基因型、HIV和丙型肝炎病毒载量以及rs12979860多态性对血清sCD40和sCD40L的影响。在HIV-1感染组中评估了CD3 +、CD4 +和CD8 +数量对sCD40和sCD40L浓度的影响。材料与方法。使用酶联免疫吸附测定法（ELISA）测定了68例丙型肝炎病毒感染患者血清中的sCD40和sCD40L浓度，其中包括39例单纯丙型肝炎病毒感染患者和29例丙型肝炎病毒/HIV-1合并感染患者。结果。与健康受试者相比，丙型肝炎病毒感染患者以及丙型肝炎病毒/HIV合并感染患者血清中sCD40和sCD40L的浓度显著更高（分别为25.7和23.2对8.5 pg/ml，以及12.7和7.3对0.79 ng/ml）。rs12979860基因型为CC的患者中sCD40L的浓度显著高于非CC基因型患者（11.8对7.6 ng/ml，p < 0.018）。结论。在慢性丙型肝炎病毒感染患者以及丙型肝炎病毒/HIV-1感染患者中检测到高水平的sCD40和sCD40L。高浓度的sCD40L与rs12979860基因型CC相关。
