Cho Su-Jin, Lee Kyeong-Lim, Kim Yu-Gon, Kim Dong-Hoon, Yoo Jae-Gyu, Yang Byoung-Chul, Park Jin-Ki, Kong Il-Keun
Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-Do, Republic of Korea.
Animal Biotechnology Division, National Institute of Animal Science, Suwon 441-706, Gyeonggi-Do, Republic of Korea.
Reprod Fertil Dev. 2016 Mar;28(3):278-85. doi: 10.1071/RD14086.
We compared the nuclear maturation status and gene-expression profiles of canine cumulus cells (CCs) derived from cumulus-oocyte complexes (COCs) that were spontaneously ovulated versus those that were matured in vitro. Cumulus-oocyte complexes were retrieved from uteri by surgical flushing (after spontaneous ovulation) or by ovariectomy follicle aspiration and in vitro maturation. The objective of Experiment 1 was to investigate the nuclear maturation status of in vivo- versus in vitro-matured oocytes. The objective of Experiment 2 was to compare gene-expression profiles of CCs derived from in vivo- versus in vitro-matured COCs. Genes analysed are related to cell maturation, development and apoptosis, including GDF9, MAPK1, PTX3, CX43, Bcl2 and BAX; mRNA expression for all of these genes, except for GDF9, differed (P<0.05) between in vivo- and in vitro-matured CCs. In conclusion, we found that gene-expression profiles are related to the quality of CCs and therefore posit that monitoring gene expression could be a useful strategy to guide attempts to improve in vitro culture systems.
我们比较了来自自发排卵的卵丘-卵母细胞复合体（COC）的犬卵丘细胞（CCs）与体外成熟的卵丘-卵母细胞复合体的核成熟状态和基因表达谱。通过手术冲洗（自发排卵后）或卵巢切除术卵泡抽吸及体外成熟从子宫中获取卵丘-卵母细胞复合体。实验1的目的是研究体内成熟与体外成熟卵母细胞的核成熟状态。实验2的目的是比较来自体内成熟与体外成熟COC的CCs的基因表达谱。所分析的基因与细胞成熟、发育和凋亡相关，包括GDF9、MAPK1、PTX3、CX43、Bcl2和BAX；除GDF9外，所有这些基因在体内成熟和体外成熟的CCs之间的mRNA表达均存在差异（P<0.05）。总之，我们发现基因表达谱与CCs的质量相关，因此认为监测基因表达可能是指导改进体外培养系统的有用策略。
