Trivellini Alice, Gordillo Belén, Rodríguez-Pulido Francisco J, Borghesi Eva, Ferrante Antonio, Vernieri Paolo, Quijada-Morín Natalia, González-Miret M Lourdes, Heredia Francisco J
Dept. Agricultural and Environmental Sciences - Production, Landscape, Agronenergy, Università degli Studi di Milano , via Celoria 2, 20133 Milan, Italy.
J Agric Food Chem. 2014 Jul 23;62(29):6966-74. doi: 10.1021/jf502444u. Epub 2014 Jul 11.
The effect of salt stress (200 mM NaCl for 28 days) on physiological characteristics of Hibiscus rosa-sinensis, such as abscisic acid (ABA) content, electrolyte leakage, and photochemical efficiency in leaves, and its influence on biomass production, anthocyanin composition, and color expression of flowers were evaluated. Salinity significantly increased electrolyte leakage and ABA content in leaves and reduced the flower fresh weight. Chlorophyll fluorescence parameters were lower in salt stress condition, compared to control. Moreover, salt stress negatively affected the content of anthocyanins (mainly cyanidin-3-sophoroside), which resulted in a visually perceptible loss of color. The detailed anthocyanin composition monitored by HPLC-DAD-MS and the color variations by digital image analysis due to salt stress showed that the effect was more noticeable at the basal portion of petals. A forward stepwise multiple regression was performed for predicting the content of anthocyanins from appearance characteristics obtained by image analysis, reaching R-square values up to 0.90.
评估了盐胁迫（200 mM NaCl处理28天）对朱槿生理特性的影响，如叶片中的脱落酸（ABA）含量、电解质渗漏和光化学效率，以及对生物量生产、花青素组成和花朵颜色表现的影响。盐度显著增加了叶片中的电解质渗漏和ABA含量，并降低了花朵鲜重。与对照相比，盐胁迫条件下叶绿素荧光参数较低。此外，盐胁迫对花青素（主要是矢车菊素-3-槐糖苷）的含量产生负面影响，导致肉眼可见的颜色损失。通过HPLC-DAD-MS监测的详细花青素组成以及盐胁迫导致的数字图像分析颜色变化表明，这种影响在花瓣基部更为明显。进行了向前逐步多元回归，以根据图像分析获得的外观特征预测花青素含量，决定系数高达0.90。
