Oloyede O B, Abalaka J A
Department of Biochemistry, University of Ilorin, Nigeria.
Microbios. 1989;57(230):49-63.
The effect of metabisulphite on the sporulating ability of Bacillus subtilis E52 and B. cereus W18 was studied. Whereas metabisulphite concentrations of 500 and 600 micrograms ml-1 prevented sporulation of B. subtilis and B. cereus, respectively, lower concentrations caused reductions in their percentage sporulations. Glucose dehydrogenase (GDH) and alkaline phosphatase (ALP) activities of both sporulating bacteria were not detected at the stated metabisulphite concentrations. A relationship between the percentage sporulation of the bacteria and the activity of the enzymes in the presence of metabisulphite was exhibited. ALP activity may serve as an index of the effectiveness of the antisporulating activity of metabisulphite. Both enzymes are likely to be among the few targets for the antisporulating activity of metabisulphite.
研究了焦亚硫酸盐对枯草芽孢杆菌E52和蜡样芽孢杆菌W18产孢能力的影响。500微克/毫升和600微克/毫升的焦亚硫酸盐浓度分别可阻止枯草芽孢杆菌和蜡样芽孢杆菌产孢，较低浓度则会降低它们的产孢百分比。在所述焦亚硫酸盐浓度下，两种产孢细菌的葡萄糖脱氢酶（GDH）和碱性磷酸酶（ALP）活性均未检测到。焦亚硫酸盐存在时，细菌的产孢百分比与酶活性之间呈现出一种关系。碱性磷酸酶活性可能作为焦亚硫酸盐抗产孢活性有效性的一个指标。这两种酶很可能是焦亚硫酸盐抗产孢活性的少数作用靶点。
