基于无标记电化学免疫传感器的动物源性食品中卡那霉素的超灵敏检测
Ultrasensitive detection of kanamycin in animal derived foods by label-free electrochemical immunosensor.
作者信息
Wei Qin, Zhao Yanfang, Du Bin, Wu Dan, Li He, Yang Minghui
机构信息
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China; School of Medical and Life Sciences, University of Jinan, Jinan 250022, China.
出版信息
Food Chem. 2012 Oct 1;134(3):1601-6. doi: 10.1016/j.foodchem.2012.02.126. Epub 2012 Mar 1.
A label-free amperometric immunosensor based on graphene sheet(GS)-nafion(Nf)/thionine(TH)/Pt nanoparticles modified electrode is proposed for the ultrasensitive detection of kanamycin. The anti-kanamycin antibody was immobilised onto the GS-Nf/TH/Pt modified glassy carbon electrode (GCE) through electrostatic adsorption. TH was selected as an electron transfer mediator, and the synergistic effects among GS, TH and Pt were investigated. The electroactivity of TH was greatly improved in the presence of GS and Pt due to their good electron-transfer ability. The proposed immunosensor shows good performance such as low detection limit (5.74 pg/mL), wide linear range (from 0.01 to 12.0 ng/mL), high stability, and good selectivity in the detection of kanamycin. The reliability of the developed immunosensor was proved by determination of kanamycin in animal derived foods with satisfactory results.
提出了一种基于石墨烯片（GS）- 全氟磺酸（Nf）/硫堇（TH）/铂纳米颗粒修饰电极的无标记电流型免疫传感器，用于超灵敏检测卡那霉素。抗卡那霉素抗体通过静电吸附固定在GS-Nf/TH/Pt修饰的玻碳电极（GCE）上。选择TH作为电子传递介质，并研究了GS、TH和Pt之间的协同效应。由于GS和Pt具有良好的电子转移能力，在它们存在的情况下，TH的电活性得到了极大提高。所提出的免疫传感器在检测卡那霉素时表现出良好的性能，如低检测限（5.74 pg/mL）、宽线性范围（0.01至12.0 ng/mL）、高稳定性和良好的选择性。通过测定动物源性食品中的卡那霉素，证明了所开发免疫传感器的可靠性，结果令人满意。
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