Albarella J P, Minegar R L, Patterson W L, Dattagupta N, Carlson E
Miles Inc., Elkhart, IN 46515.
Nucleic Acids Res. 1989 Jun 12;17(11):4293-308. doi: 10.1093/nar/17.11.4293.
We describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. These compounds were used to label nucleic acids in the presence of lysed cell constituents. The resulting labelled nucleic acids show hybridization to a genus specific probe for E. coli. The relative comparison of sensitivity indicates that a polyamine linker is better than a polyethylene oxide linker between the biotin and angelicin moieties.
我们描述了三种可用于用生物素标记核酸的当归素衍生物的合成。这些化合物用于在裂解的细胞成分存在下标记核酸。所得的标记核酸与大肠杆菌属特异性探针杂交。灵敏度的相对比较表明，在生物素和当归素部分之间，多胺连接体比聚环氧乙烷连接体更好。
