Althaus Denise, Lehner Angelika, Brisse Sylvain, Maury Mylène, Tasara Taurai, Stephan Roger
1 Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich , Zurich, Switzerland .
Foodborne Pathog Dis. 2014 Oct;11(10):753-8. doi: 10.1089/fpd.2014.1747. Epub 2014 Jul 9.
Listeria monocytogenes, an emerging foodborne pathogen, can cause in the population at risk severe infections that are associated with high case fatality rates. A total of 93 L. monocytogenes strains isolated from different patients in Switzerland from July 2011 to September 2013 were further characterized. Septicemia was reported for 74.2% of the patients, meningitis for 10.8%, and abortion for 3.2%. The majority of the strains belonged to serotype 1/2a (n=58) followed by serotype 4b (n=28), 1/2b (n=5), and 1/2c (n=2). The strains represented 35 multilocus sequence typing sequence types, 8 of which were designated for the first time. Sequence analysis of the inlA gene in the 35 sequence types showed that most of the strains encoded full-length proteins. Screening for Listeriolysin S showed the presence of this virulence factor in 29 of the 33 genetic lineage I strains. By using ApaI and AscI for pulsed-field gel electrophoresis, most strains showed distinguishable patterns.
单核细胞增生李斯特菌是一种新出现的食源性病原体，可在高危人群中引起与高病死率相关的严重感染。对2011年7月至2013年9月期间从瑞士不同患者中分离出的93株单核细胞增生李斯特菌进行了进一步鉴定。报告显示，74.2%的患者发生败血症，10.8%的患者发生脑膜炎，3.2%的患者发生流产。大多数菌株属于血清型1/2a（n = 58），其次是血清型4b（n = 28）、1/2b（n = 5）和1/2c（n = 2）。这些菌株代表了35种多位点序列分型序列类型，其中8种是首次确定。对35种序列类型的inlA基因进行序列分析表明，大多数菌株编码全长蛋白。对李斯特菌溶血素S的筛查显示，33株遗传谱系I菌株中有29株存在这种毒力因子。通过使用ApaI和AscI进行脉冲场凝胶电泳，大多数菌株呈现出可区分的图谱。
