急性黄曲霉毒素B1处理后大鼠脑内色氨酸、5-羟色胺和酪氨酸水平的差异变化。

Differential changes in rat brain tryptophan, serotonin and tyrosine levels following acute aflatoxin B1 treatment.

作者信息

Weekley L B, O'Rear C E, Kimbrough T D, Llewellyn G C

机构信息

School of Veterinary Medicine, Colorado State University, Fort Collins.

出版信息

Toxicol Lett. 1989 May;47(2):173-7. doi: 10.1016/0378-4274(89)90072-6.

DOI:10.1016/0378-4274(89)90072-6

PMID:2500755

Abstract

Female Sprague-Dawley rats were treated acutely (12-h) with aflatoxin B1 (100 micrograms/kg i.p.) or vehicle (10% acetone in 0.9% NaCl) and regional brain levels of tryptophan, serotonin and tyrosine were assayed. Brainstem but not cerebellar or cortical tyrosine levels were decreased in aflatoxin B1-treated rats. Brain tryptophan was increased in all 3 brain regions by acute aflatoxin B1 treatment, while serotonin levels were unaltered in the cerebellum and cortex and decreased in the brainstem. These experiments indicate that acute aflatoxin B1 treatment differentially alters brain amino acids and serotonin and that changes in brain tryptophan, the serotonin precursor, do not parallel changes in brain serotonin.

摘要

将雌性斯普拉格-道利大鼠用黄曲霉毒素B1（100微克/千克，腹腔注射）或赋形剂（0.9%氯化钠中的10%丙酮）进行急性处理（12小时），并测定大脑各区域色氨酸、5-羟色胺和酪氨酸的水平。在经黄曲霉毒素B1处理的大鼠中，脑干的酪氨酸水平降低，但小脑和皮质的酪氨酸水平未降低。急性黄曲霉毒素B1处理使所有3个脑区的脑色氨酸增加，而小脑和皮质中的5-羟色胺水平未改变，脑干中的5-羟色胺水平降低。这些实验表明，急性黄曲霉毒素B1处理可不同程度地改变脑氨基酸和5-羟色胺，且5-羟色胺前体色氨酸在脑中的变化与脑5-羟色胺的变化并不平行。