Zhou Li-Na, Yu Hai-Ye, Zhang Lei, Ren Shun, Sui Yuan-Yuan, Yu Lian-Jun
Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Apr;34(4):1003-6.
In order to detect rice blast more rapidly, accurately and nondestructively, the identification and early warning models of rice blast were established in the present research. First of all, rice blast was divided into three grades according to the relative area of disease spots in rice leaf and laser induced chlorophyll fluorescence spectra of rice leaves at different disease levels were measured in the paddy fields. Meanwhile, 502-830 nm bands of laser-induced chlorophyll fluorescence spectra were selected for the study of rice blast. Savitzky-Golay(SG) smoothing and First Derivative Transform(FDT) were applied for the pretreatment of laser-induced chlorophyll fluorescence spectra. Then the method of Principal Components Analysis (PCA) was used to achieve the dimension reduction on spectral information, three principal components whose variance are greater than 1 and cumulative credibility is 99.924% were extracted by this method. Furthermore, the tentative data were divided into calibration set and validation set, the levels of rice blast were taken as the predictors. Combined with the calibration set which contains the disease and spectral information of 133 leaves, Discriminant Analysis (DA), Multiple Logistic Regression Analysis (MLRA) and Multilayer Perceptron (MLP) were used respectively to establish the identification and early warning models of rice blast. The Prediction examinations of the three models were made based on the validation set which contains the disease and spectral information of 89 leaves. The results show that all the models of PCA-DA, PCA-MLRA and PCA-MLP can carry on the prediction of rice blast, and the average prediction accuracy of PCA-MLP prediction model is 91.7% which is improved compared with PCA- DA and PCA- MLRA.
为了更快速、准确且无损地检测稻瘟病,本研究建立了稻瘟病的识别与预警模型。首先,根据水稻叶片病斑相对面积将稻瘟病分为三个等级,并在稻田中测定了不同病情水平下水稻叶片的激光诱导叶绿素荧光光谱。同时,选取激光诱导叶绿素荧光光谱的502 - 830 nm波段用于稻瘟病研究。采用Savitzky - Golay(SG)平滑和一阶导数变换(FDT)对激光诱导叶绿素荧光光谱进行预处理。然后运用主成分分析(PCA)方法对光谱信息进行降维,通过该方法提取出方差大于1且累积贡献率为99.924%的三个主成分。此外,将试验数据分为校正集和验证集,以稻瘟病等级作为预测变量。结合包含133片叶片病情和光谱信息的校正集,分别使用判别分析(DA)、多元逻辑回归分析(MLRA)和多层感知器(MLP)建立稻瘟病的识别与预警模型。基于包含89片叶片病情和光谱信息的验证集对这三个模型进行预测检验。结果表明,PCA - DA、PCA - MLRA和PCA - MLP模型均能对稻瘟病进行预测,其中PCA - MLP预测模型的平均预测准确率为91.7%,与PCA - DA和PCA - MLRA相比有所提高。