Persia Fabio Andrés, Mariani María Laura, Fogal Teresa Hilda, Penissi Alicia Beatriz
Instituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Argentina.
Instituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Argentina.
Phytomedicine. 2014 Sep 25;21(11):1400-5. doi: 10.1016/j.phymed.2014.05.010. Epub 2014 Jul 6.
The aim of this study was to determine whether hydroxytyrosol and oleuropein, the major phenols found in olives and olive oil, inhibit mast cell activation induced by immune and non-immune pathways. Purified peritoneal mast cells were preincubated in the presence of test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin A, compound 48/80 or calcium ionophore A23187. Dose-response and time-dependence studies were carried out. Comparative studies with sodium cromoglycate, a classical mast cell stabilizer, were also made. After incubation the supernatants and pellets were used to determine the β-hexosaminidase content by colorimetric reaction. The percentage of β-hexosaminidase release in each tube was calculated and taken as a measure of mast cell activation. Other samples of cell pellets were used for cell viability studies by the trypan blue dye exclusion test, or fixed for light and electron microscopy. Biochemical and morphological findings of the present study showed for the first time that hydroxytyrosol and oleuropein inhibit mast cell degranulation induced by both immune and non-immune pathways. These results suggest that olive phenols, particularly hydroxytyrosol and oleuropein, may provide insights into the development of useful tools for the prevention and treatment of mast cell-mediated disorders.
本研究的目的是确定橄榄和橄榄油中的主要酚类物质羟基酪醇和橄榄苦苷是否能抑制免疫和非免疫途径诱导的肥大细胞活化。在与伴刀豆球蛋白A、化合物48/80或钙离子载体A23187孵育之前，将纯化的腹膜肥大细胞在受试化合物（羟基酪醇或橄榄苦苷）存在下进行预孵育。进行了剂量反应和时间依赖性研究。还与经典的肥大细胞稳定剂色甘酸钠进行了比较研究。孵育后，使用上清液和沉淀通过比色反应测定β-己糖胺酶含量。计算每管中β-己糖胺酶的释放百分比，并将其作为肥大细胞活化的指标。细胞沉淀的其他样本样本用于样品用于通过台盼蓝染料排除试验进行细胞活力研究，或固定用于光镜和电镜观察。本研究的生化和形态学结果首次表明，羟基酪醇和橄榄苦苷可抑制免疫和非免疫途径诱导的肥大细胞脱颗粒。这些结果表明，橄榄酚类物质，特别是羟基酪醇和橄榄苦苷，可能为开发预防和治疗肥大细胞介导疾病的有用工具提供思路。
