Gábriel R, Fekete E, Halasy K
Department of Zoology, Attila József University, Szeged, Hungary.
Acta Histochem. 1989;85(2):135-41. doi: 10.1016/S0065-1281(89)80056-X.
An investigation was made on the frog stomach myenteric plexus with 2 different histochemical techniques. Neuronal perikarya were stained with nicotinamide-adenine-dinucleotide-diaphorase (NADHd), while the acetyl-cholinesterase (AChE) staining showed rather the axoarchitectonic arrangement of the frog myenteric plexus. In double-labelled "whole mounts", NADHd-positive cell bodies and AChE-positive nerve processes were revealed. Some of the nerve cells and neuronal processes did not exhibit AChE activity at all. Since glyoxylic acid-induced fluorescence (GIF) was not detected in the myenteric plexus, the presence of catecholamines can be excluded. As a consequence of these observations, we suggest the presence of a non-cholinergic, non-adrenergic intrinsic neuronal system in the frog stomach myenteric plexus, containing purines or peptides as transmitters.
采用两种不同的组织化学技术对蛙胃肌间神经丛进行了研究。神经元胞体用烟酰胺腺嘌呤二核苷酸黄递酶（NADHd）染色，而乙酰胆碱酯酶（AChE）染色则显示了蛙肌间神经丛的轴突构筑排列。在双重标记的“整装片”中，发现了NADHd阳性细胞体和AChE阳性神经突起。一些神经细胞和神经突起根本没有表现出AChE活性。由于在肌间神经丛中未检测到乙醛酸诱导荧光（GIF），因此可以排除儿茶酚胺的存在。基于这些观察结果，我们认为蛙胃肌间神经丛中存在一个非胆碱能、非肾上腺素能的内在神经元系统，其含有嘌呤或肽作为神经递质。
