Li Shuhua, Tang Xian, Qian Xiaohua, Yuan Zheng'an, Li Chongshan, Yang Yanji, Yu Xiaonan, Gong Xiangzhen
Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
Shanghai Municipal Center for Disease Control and Prevention.
Zhonghua Liu Xing Bing Xue Za Zhi. 2014 Apr;35(4):429-32.
To ascertain the genotype of measles viruses isolated in 2012 and genetic characterization of measles viruses in Hongkou district of Shanghai during 2000-2012.
Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus on nucleoprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain announced by GenBank during 2000-2012. Measles virus genotype was analyzed. Epidemiological investigation was conducted.
Phylogenetic analysis showed that 7 measles virus samples were isolated from 34 throat swab specimens with 6 of them belonged to H1 genotype, 1 belonged to D8 genotype of H1 genotype. H1a appeared the main part of Shanghai measles virus. Epidemiological survey showed that D8 was an imported case, also the first case detected since 2000.
The genotype distribution of measles virus in Hongkou was identified the same as elsewhere in Shanghai. D8 was an imported case, detected for the first time since 2000. The results suggested that viral gene sequencing and genotyping should be regularly conducted at the measles laboratories in Shanghai to strengthen the networking monitoring program of the disease.
确定2012年分离的麻疹病毒基因型以及2000 - 2012年上海市虹口区麻疹病毒的基因特征。
从疑似麻疹病例的咽拭子标本中分离麻疹病毒，采用逆转录聚合酶链反应（RT-PCR）扩增核蛋白（N）基因C端450bp片段。进行序列分析以确定基因型，并与GenBank公布的2000 - 2012年其他麻疹病毒株进行核苷酸差异比较。分析麻疹病毒基因型，并开展流行病学调查。
系统发育分析显示，从34份咽拭子标本中分离出7株麻疹病毒，其中6株属于H1基因型，1株属于H1基因型的D8基因型。H1a是上海麻疹病毒的主要组成部分。流行病学调查显示，D8为输入性病例，也是2000年以来首次检测到的病例。
虹口区麻疹病毒的基因型分布与上海其他地区一致。D8是输入性病例，为2000年以来首次检测到。结果提示，上海市麻疹实验室应定期开展病毒基因测序和基因分型工作，以加强该病的网络监测方案。
