Cui Ning, Su Shuai, Chen Zimeng, Zhao Xiaomin, Cui Zhizhong
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, PR China.
College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
J Gen Virol. 2014 Nov;95(Pt 11):2512-2522. doi: 10.1099/vir.0.067264-0. Epub 2014 Jul 9.
The strain JS11C1, a member of a putative new subgroup of avian leukosis virus (ALV) that is different from all six known subgroups from chickens based on Gp85 amino acid sequence comparison, was isolated from Chinese native chicken breeds in 2012. In order to further study the genome structure, biological characteristics, and the evolutionary relationship of the virus with others of known subgroups from infected chickens, we determined the complete genome sequence, constructed an infectious clone of ALV strain JS11C1, and performed comparative analysis using the whole genome sequence or elements with that of other ALVs available in GenBank. The results showed that the full-length sequence of the JS11C1 DNA provirus genome was 7707 bp, which is consistent with a genetic organization typical of a replication-competent type C retrovirus lacking viral oncogenes. The rescued infectious clone of JS11C1 showed similar growth rate and biological characteristics to its original virus. All the comparison analyses based on whole genomes support the opinion that the new isolates are relatively distantly related to any known subgroups of ALVs and might be classified as a new subgroup.
JS11C1毒株于2012年从中国本土鸡品种中分离得到，基于Gp85氨基酸序列比较，它属于禽白血病病毒（ALV）一个假定新亚群的成员，与鸡的所有六个已知亚群均不同。为了进一步研究该病毒的基因组结构、生物学特性以及与感染鸡中已知亚群其他病毒的进化关系，我们测定了ALV毒株JS11C1的全基因组序列，构建了其感染性克隆，并使用全基因组序列或元件与GenBank中其他ALV的序列进行了比较分析。结果表明，JS11C1 DNA前病毒基因组的全长序列为7707 bp，这与缺乏病毒癌基因的具有复制能力的C型逆转录病毒的典型基因结构一致。拯救的JS11C1感染性克隆显示出与其原始病毒相似的生长速率和生物学特性。基于全基因组的所有比较分析均支持以下观点：新分离株与任何已知的ALV亚群关系相对较远，可能被归类为一个新亚群。
