Kokoszko-Bilska Agnieszka, Stepniak Jan, Lewinski Andrzej, Karbownik-Lewinska Malgorzata
Department of Oncological Endocrinology, Medical University of Lodz, 7/9 Zeligowski St, Lodz 90-752, Poland ; Polish Mother's Memorial Hospital - Research Institute, 281/289, Rzgowska St, Lodz 93-338, Poland.
Department of Oncological Endocrinology, Medical University of Lodz, 7/9 Zeligowski St, Lodz 90-752, Poland.
Thyroid Res. 2014 Jun 5;7:5. doi: 10.1186/1756-6614-7-5. eCollection 2014.
Whereas oxidative reactions occur in all tissues and organs, the thyroid constitutes such an organ, in which oxidative processes are indispensable for physiological functions. In turn, numerous metabolic reactions occurring in the liver create favourable conditions for huge oxidative stress. Melatonin is a well-known antioxidant with protective effects against oxidative damage perfectly documented in many tissues, the thyroid and the liver included. Caffeic acid phenethyl ester (CAPE), a component of honeybee propolis, has been suggested to be also an effective antioxidant. The aim of the study was to evaluate the effects of CAPE on Fenton reaction-induced oxidative damage to membrane lipids (lipid peroxidation, LPO) in porcine thyroid and liver, and to compare the results with protective effects of melatonin.
Thyroid and liver homogenates were incubated in the presence of CAPE (500; 100; 50; 10; 5.0; 1.0 μM) or melatonin (500; 100; 50; 10; 5.0; 1.0 μM), without or with addition of FeSO4 (30 μM) + H2O2 (0.5 mM). The level of lipid peroxidation was measured spectrophotometrically and expressed as the amount of MDA + 4-HDA (nmol) per mg of protein.
Whereas CAPE decreased the basal LPO in a concentration-dependent manner in both tissues, melatonin did not change the basal LPO level. When antioxidants were used together with Fenton reaction substrates, they prevented - in a concentration-dependent manner and to a similar extent - experimentally-induced LPO in both tissues.
Protective antioxidative effects of CAPE in the thyroid and the liver are similar to those caused by melatonin. CAPE constitutes a promising agent in terms of its application in experimental and, possibly, clinical studies.
氧化反应发生于所有组织和器官，甲状腺就是这样一个器官，其中氧化过程对生理功能而言不可或缺。反过来，肝脏中发生的众多代谢反应为巨大的氧化应激创造了有利条件。褪黑素是一种著名的抗氧化剂，其对氧化损伤的保护作用在包括甲状腺和肝脏在内的许多组织中都有充分记录。咖啡酸苯乙酯（CAPE）是蜜蜂蜂胶的一种成分，也被认为是一种有效的抗氧化剂。本研究的目的是评估CAPE对芬顿反应诱导的猪甲状腺和肝脏膜脂质氧化损伤（脂质过氧化，LPO）的影响，并将结果与褪黑素的保护作用进行比较。
甲状腺和肝脏匀浆在有CAPE（500；100；50；10；5.0；1.0 μM）或褪黑素（500；100；50；10；5.0；1.0 μM）存在的情况下孵育，不添加或添加FeSO4（30 μM）+ H2O2（0.5 mM）。脂质过氧化水平通过分光光度法测定，并表示为每毫克蛋白质中MDA + 4 - HDA的量（nmol）。
虽然CAPE在两种组织中均以浓度依赖的方式降低基础LPO，但褪黑素并未改变基础LPO水平。当抗氧化剂与芬顿反应底物一起使用时，它们以浓度依赖的方式并在相似程度上防止了两种组织中实验诱导的LPO。
CAPE在甲状腺和肝脏中的保护抗氧化作用与褪黑素引起的作用相似。就其在实验以及可能的临床研究中的应用而言，CAPE是一种有前景的药物。
