人胎盘微绒毛作为抗原来源用于制备抗低密度脂蛋白受体的多克隆抗体。
Human placental microvilli as a source of antigen for the preparation of a polyclonal antibody directed against the LDL receptor.
作者信息
Goldstein S, Moreau M, Barbu V, Meier M, Alsat E, Mazière C, Mazière J C, Polonovski J
机构信息
Laboratoire de Biochimie, Faculté de Médecine Saint-Antoine, Paris, France.
出版信息
Biochimie. 1989 Mar;71(3):379-82. doi: 10.1016/0300-9084(89)90010-2.
Polyclonal antibodies were prepared by immunization of rabbits with partially purified LDL receptor obtained from human placental microvilli. The antiserum reacted with membranes from human placental microvilli and human fibroblasts, as assessed by immunobinding studies. It also reacted with purified LDL receptors of both origins. The antiserum markedly inhibited 125I-labeled LDL binding to cultured human fibroblasts.
通过用从人胎盘微绒毛中获得的部分纯化的低密度脂蛋白（LDL）受体免疫兔子来制备多克隆抗体。通过免疫结合研究评估，该抗血清与人胎盘微绒毛和人成纤维细胞的膜发生反应。它也与两种来源的纯化LDL受体发生反应。该抗血清显著抑制125I标记的LDL与培养的人成纤维细胞的结合。
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