Fukumoto H, Matsui Y, Obinata M
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Cell Struct Funct. 1989 Apr;14(2):231-9. doi: 10.1247/csf.14.231.
The murine erythroleukemia (MEL) cell line, TSA8, becomes responsive to erythropoietin after induction with dimethyl sulfoxide (DMSO). We examined the signalling pathways involved in the commitment of TSA8 cells to become the erythroid progenitor cells responsive to erythropoietin, comparing them with the pathway used in an erythropoietin-induced change of the progenitor cells. Amiloride, an inhibitor of the Na+/H+ antiporter, completely blocked the commitment of TSA8 cells to become responsive to erythropoietin at a concentration that did not affect cell proliferation, while it showed no effect on the differentiation or proliferation of the erythroid progenitor cells derived from TSA8 cells by erythropoietin. Ethyleneglycol-bis (beta-aminoethyl ether) N,N,N',N'-tetra acetic acid (EGTA) inhibited the commitment of TSA8 cells to CFU-E-like cells without affecting colony formation. In contrast, EGTA did not inhibit erythropoietin-induced differentiation of the progenitor cells, but did inhibit their proliferation. These results indicate that erythropoietin uses different signalling pathways from those used in the induction of the commitment of TSA8 cells.
小鼠红白血病（MEL）细胞系TSA8在用二甲基亚砜（DMSO）诱导后对促红细胞生成素产生反应。我们研究了TSA8细胞向对促红细胞生成素产生反应的红系祖细胞定向分化过程中涉及的信号通路，并将其与促红细胞生成素诱导祖细胞发生变化所使用的信号通路进行了比较。氨氯地平是一种Na+/H+反向转运蛋白抑制剂，在不影响细胞增殖的浓度下，它完全阻断了TSA8细胞对促红细胞生成素产生反应的定向分化，而对促红细胞生成素诱导的TSA8来源的红系祖细胞的分化或增殖没有影响。乙二醇双（β-氨基乙基醚）N，N，N'，N'-四乙酸（EGTA）抑制TSA8细胞向CFU-E样细胞的定向分化，而不影响集落形成。相反，EGTA不抑制促红细胞生成素诱导的祖细胞分化，但抑制其增殖。这些结果表明，促红细胞生成素使用的信号通路与诱导TSA8细胞定向分化所使用的信号通路不同。
