He Xiaojun, Li Jia, Zhang Hong, Tan Lifeng
College of Chemistry, Xiangtan University, Xiangtan 411105, China.
Mol Biosyst. 2014 Oct;10(10):2552-7. doi: 10.1039/c4mb00304g.
There is renewed interest in investigating triplex nucleic acids because triplexes may be implicated in a range of cellular functions. However, the stabilization of triplex nucleic acids is essential to achieve their biological functions. In contrast to triplex DNA, little has been reported concerning the recognition of triplex RNA by transition-metal complexes at present. We report here a ruthenium(ii) polypyridyl complex, Ru(bpy)2(mdpz) (bpy = 2,2'-bipyridine; mdpz = 7,7'-methylenedioxyphenyl-dipyrido-[3,2-a:2',3'-c]phenazine), as a sensitive luminescent probe for poly(U)·poly(A)*poly(U), which can strongly stabilize the triplex RNA from 37.5 to 53.1 °C in solution. The main results further advance our knowledge on the triplex RNA-binding by metal complexes, particularly ruthenium(ii) complexes.
由于三链核酸可能参与一系列细胞功能，因此对其研究重新引起了人们的兴趣。然而，三链核酸的稳定对于实现其生物学功能至关重要。与三链DNA不同，目前关于过渡金属配合物对三链RNA的识别报道较少。我们在此报告一种钌（II）多吡啶配合物Ru(bpy)2(mdpz)（bpy = 2,2'-联吡啶；mdpz = 7,7'-亚甲基二氧苯基-二吡啶并-[3,2-a:2',3'-c]吩嗪），作为聚（U）·聚（A）*聚（U）的灵敏发光探针，它能在溶液中将三链RNA的稳定温度从37.5℃提高到53.1℃。这些主要结果进一步加深了我们对金属配合物，特别是钌（II）配合物与三链RNA结合的认识。
