不同纤维类型组成的大鼠和家兔骨骼肌中对乙酰唑胺敏感和耐药的碳酸酐酶活性

Acetazolamide-sensitive and resistant carbonic anhydrase activity in rat and rabbit skeletal muscles of different fiber type composition.

Frémont P, Boudriau S, Tremblay R R, Côté C, Rogers P A

Muscle Biology Research Group, Laval University Hospital Research Centre, St-Foy, Québec, Canada.

Int J Biochem. 1989;21(2):143-7. doi: 10.1016/0020-711x(89)90102-x.

Abstract

Acetazolamide (ACET)-resistant and -sensitive carbonic anhydrase (CA) activity was measured in post-mitochondrial supernatants from the soleus (SOL), deep vastus lateralis (DVL) and superficial vastus lateralis (SVL) muscles of rats and rabbits. 2. The relative total CA activity in the three muscles of both species can be summarized as SOL greater than DVL greater than SVL. 3. ACET-resistant CA activity was found in the SOL and DVL muscles of both species whereas a low level of ACET-sensitive CA activity was detected in the SVL muscle. 4. ACET-sensitive CA activity was also found in sarcoplasmic reticulum preparations from rat and rabbit SOL muscles.

摘要

测定了大鼠和家兔比目鱼肌（SOL）、股外侧肌深部（DVL）和股外侧肌浅部（SVL）线粒体后上清液中对乙酰唑胺（ACET）耐药和敏感的碳酸酐酶（CA）活性。2. 两种动物的这三块肌肉中相对总CA活性可概括为：SOL＞DVL＞SVL。3. 在两种动物的SOL和DVL肌肉中均发现了对ACET耐药的CA活性，而在SVL肌肉中检测到低水平的对ACET敏感的CA活性。4. 在大鼠和家兔SOL肌肉的肌浆网制剂中也发现了对ACET敏感的CA活性。