Li Jin-Xia, Zhang Man, Sun Li-Bo, Zhang Ling, Zhang Wen-Quan, Zhao Hai-Fu, Li Peng-Ling, Hua Yong-Li, Ji Peng, Wei Yan-Ming
Zhongguo Zhong Yao Za Zhi. 2014 Apr;39(7):1293-9.
Metabonomics was employed to investigate the effect of Angelica sinensis volatile oil (ASVO) to the endogenous metabolites of normal rats, and to reveal the possible ways of metabolism in rats caused by ASVO. The fifty male Waster rats were randomly divided into five groups (each consists of 10 rats), such as control group, high dose group of ASVO, middle dose group of ASVO, low dose group of ASVO, and Aspirin group. They were given 0.9% saline, 0.352 mL x kg(-1) ASVO, 0.176 mL x kg(-1) ASVO, 0.088 mL x kg(-1) ASVO and ASP respectively with the equal volume of 0.2 mL. Drugs and vehicle were given for 3 successive days. The urine was collected at 12, 24, 36, 48 h after modeling with metabolic cages. Rat urine metabolic fingerprint in different stages was analyzed using GC-MS, based on which the principal component analysis (PCA)and orthogonal partial least-squares discriminant analysis (OPLS-DA) models were established for metabonomic analysis. Potential biomarkers were screened by using variable importance in the projection (VIP) and T test. It was revealed that the middle dose of ASVO at 36 h induces a substantial change in rat urine. Compared with control group, seven kinds of endogenous metabolites in ASP group and ASVO group change significantly (P < 0.05), among which aconitic acid, succinic acid, citric acid, alpha-ketone glutaric acid, glycine and malic acid content had an upward trend (P < 0.05) and prostaglandin content had a downward trend (P < 0.01). The mechanism of ASVO and ASP have the similarity. It is likely that ASVO intervenes the metabolic process by affecting the energy, amino acid and lipid metabolism. Our work also indicates that rats administrated with ASVO can increase the energy metabolism of the body, induce the production of inflammatory substances and strengthen the body's immune ability. The result has also provide a proof for futher interpret ASVO pharmacological effects.
采用代谢组学方法研究当归挥发油（ASVO）对正常大鼠内源性代谢物的影响，并揭示ASVO在大鼠体内可能的代谢途径。将50只雄性Waster大鼠随机分为5组（每组10只），即对照组、ASVO高剂量组、ASVO中剂量组、ASVO低剂量组和阿司匹林组。分别给予0.9%生理盐水、0.352 mL·kg⁻¹ ASVO、0.176 mL·kg⁻¹ ASVO、0.088 mL·kg⁻¹ ASVO和ASP，体积均为0.2 mL。连续给药3天。用代谢笼在造模后12、24、36、48 h收集尿液。采用气相色谱-质谱联用仪（GC-MS）分析大鼠不同阶段尿液代谢指纹图谱，在此基础上建立主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA）模型进行代谢组学分析。通过变量重要性投影（VIP）和T检验筛选潜在生物标志物。结果表明，ASVO中剂量在36 h时可引起大鼠尿液显著变化。与对照组相比，ASP组和ASVO组7种内源性代谢物变化显著（P<0.05），其中乌头酸、琥珀酸、柠檬酸、α-酮戊二酸、甘氨酸和苹果酸含量呈上升趋势（P<0.05），前列腺素含量呈下降趋势（P<0.01）。ASVO和ASP的作用机制具有相似性。ASVO可能通过影响能量、氨基酸和脂质代谢来干预代谢过程。本研究还表明，给予ASVO的大鼠可增加机体能量代谢，诱导炎症物质产生，增强机体免疫能力。该结果也为进一步阐释ASVO的药理作用提供了依据。
