Nazian S J, Dietz J R, Rowland M D
Department of Physiology and Biophysics, College of Medicine, University of South Florida, Tampa 33612.
J Androl. 1989 May-Jun;10(3):174-9. doi: 10.1002/j.1939-4640.1989.tb00082.x.
Chronic renal insufficiency was induced in young adult male rats by a 2-step 5/6 nephrectomy. Four weeks after surgery the rats were anesthetized with ketamine and injected with saline or 1, 5, 10, 50, or 100 ng of LHRH/100 g BW and blood samples were collected at various times and assayed for LH. A second group of rats was injected with saline or 5, 10, 50, 100, or 200 ng of LHRH/100 g BW and serum samples assayed for FSH. A third experiment tested for the presence of a self-priming effect of LHRH on LH secretion. Rats were primed with three iv injections of 10 ng of LHRH/100 g BW or saline at 30-min intervals and challenged with 50 ng of LHRH/100 g BW 30 min after the last priming injection. Ten min after the challenge injection, the LH increment was determined. The data indicate that male rats suffering from chronic renal insufficiency have an impaired LH response to low doses of LHRH, essentially normal FSH responses to most doses of LHRH examined, and lack a self-priming effect of LHRH on LH secretion. These results suggest that part of the reproductive abnormalities found in chronic renal failure may be due to alterations in pituitary LH responses.
采用两步5/6肾切除术在成年雄性幼鼠中诱导慢性肾功能不全。术后四周，用氯胺酮麻醉大鼠，并注射生理盐水或1、5、10、50或100 ng促黄体生成素释放激素（LHRH）/100 g体重，在不同时间采集血样并检测促黄体生成素（LH）。第二组大鼠注射生理盐水或5、10、50、100或200 ng LHRH/100 g体重，并检测血清样本中的促卵泡生成素（FSH）。第三个实验检测LHRH对LH分泌的自我启动效应的存在情况。大鼠每隔30分钟静脉注射三次10 ng LHRH/100 g体重或生理盐水进行预刺激，并在最后一次预刺激注射后30分钟用50 ng LHRH/100 g体重进行激发。激发注射后10分钟，测定LH的增量。数据表明，患有慢性肾功能不全的雄性大鼠对低剂量LHRH的LH反应受损，对所检测的大多数剂量LHRH的FSH反应基本正常，并且LHRH对LH分泌缺乏自我启动效应。这些结果表明，慢性肾衰竭中发现的部分生殖异常可能归因于垂体LH反应的改变。
