Wolosz Dominika, Walczak Agnieszka, Wilczynski Grzegorz M, Szparecki Grzegorz, Wilczek Ewa, Gornicka Barbara
Department of Pathomorphology, Medical University of Warsaw, Warsaw, Mazovia 02-091, Poland.
Laboratory of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Mazovia 02-093, Poland.
Oncol Lett. 2014 Aug;8(2):785-788. doi: 10.3892/ol.2014.2216. Epub 2014 Jun 3.
The deleted in liver cancer (DLC) protein family is composed of proteins that are hypothesized to function predominantly by regulating the activity of the small GTPases. The aim of the present study was to determine the expression and exact localization of DLC1 in hepatocellular carcinoma (HCC) tissue sections. In two types of HCC tissues, typical and fibrolamellar, immunohistochemical and immunofluorescent analysis were performed to assess DLC1 immunoreactivity. Additionally, the gene status was determined by the fluorescence hybridization. According to the observations, DLC1 is often lost in cancer cells; however, it can remain within the stromal component of tumor sections. The DLC1 immunoreactivity was particularly noticeable within the capsules surrounding the tumor masses. It was found that the gene was deleted in 52% of HCC cases. In addition, the hemizygous deletion was observed to be independent of the HCC subtype. The results indicate that although the loss of DLC1 is a common step during hepatocarcinogenesis, this protein may be present in the tumor microenvironment.
肝癌缺失蛋白（DLC）家族由一些蛋白质组成，据推测这些蛋白质主要通过调节小GTP酶的活性发挥作用。本研究的目的是确定DLC1在肝细胞癌（HCC）组织切片中的表达及确切定位。在两种类型的HCC组织（典型型和纤维板层型）中，进行了免疫组织化学和免疫荧光分析以评估DLC1的免疫反应性。此外，通过荧光杂交确定基因状态。根据观察结果，DLC1在癌细胞中常缺失；然而，它可保留在肿瘤切片的基质成分中。DLC1免疫反应性在肿瘤块周围的包膜内尤为明显。研究发现，52%的HCC病例中该基因发生缺失。此外，观察到半合子缺失与HCC亚型无关。结果表明，尽管DLC1缺失是肝癌发生过程中的一个常见步骤，但该蛋白可能存在于肿瘤微环境中。
