Jiang Ya-Hui, Wang Ping, Yang Hong-Jian, Chen Ying
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Agro-product Safety Research Centre, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
Toxins (Basel). 2014 Jul 10;6(7):2008-23. doi: 10.3390/toxins6072008.
Two commercial materials, a bamboo charcoal (BC) and a smectite clay (SC), were assessed in vitro with aflatoxin B1 (AFB1) in an equilibrium adsorption test. The adsorption capacity and proportion adsorbed (0.381 μg/mg, 0.955) for BC were greater than for SC (0.372 μg/mg, 0.931). The effects of in vitro ruminal fermentation of hay-rich feed incubated with 1.0 μg/mL AFB1 for 0-10 g/L doses of BC and SC were measured at 39 °C for 72 h. The BC and SC binders increased AFB1 loss at dosages ≥1.0 g/L (p < 0.0001). Average AFB1 loss (p < 0.0001) was greater for SC (0.904) than BC (0.881). Both SC and SC addition increased in vitro dry matter loss, and the average dry matter losses were similar. Asymptotic gas volume and volatile fatty acid production were greater for BC than for SC (p < 0.0001). Thus, BC may be as effective as SC in removing aflatoxin B1's detrimental effects on rumen degradability and fermentation under the occurrence of microbial aflatoxin degradation.
在平衡吸附试验中，对两种商业材料——竹炭（BC）和蒙脱石粘土（SC）——进行了黄曲霉毒素B1（AFB1）的体外评估。竹炭的吸附容量和吸附比例（0.381μg/mg，0.955）高于蒙脱石粘土（0.372μg/mg，0.931）。在39℃下培养72小时，测定了富含干草的饲料与1.0μg/mL AFB1一起孵育时，0 - 10g/L剂量的竹炭和蒙脱石粘土对体外瘤胃发酵的影响。当剂量≥1.0g/L时，竹炭和蒙脱石粘土结合剂增加了AFB1的损失（p < 0.0001）。蒙脱石粘土的平均AFB1损失（p < 0.0001）（0.904）高于竹炭（0.881）。添加竹炭和蒙脱石粘土均增加了体外干物质损失，且平均干物质损失相似。竹炭的渐近气体体积和挥发性脂肪酸产量高于蒙脱石粘土（p < 0.0001）。因此，在微生物降解黄曲霉毒素的情况下，竹炭在消除黄曲霉毒素B1对瘤胃降解性和发酵的有害影响方面可能与蒙脱石粘土一样有效。
