广西壮族人群雌激素受体α基因中PvuII和XbaI多态性与乙型肝炎病毒感染风险的关联
Association of PvuII and XbaI polymorphisms in estrogen receptor alpha gene with the risk of hepatitis B virus infection in the Guangxi Zhuang population.
作者信息
Liu Yanqiong, Liu Yan, Huang Xiamei, Sui Jingzhe, Mo Cuiju, Wang Jian, Peng Qiliu, Deng Yan, Huang Li, Li Shan, Qin Xue
机构信息
Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
出版信息
Infect Genet Evol. 2014 Oct;27:69-76. doi: 10.1016/j.meegid.2014.07.002. Epub 2014 Jul 9.
BACKGROUND AND OBJECTIVE
Available evidence has suggested that estrogen receptor alpha (ESR1) is implicated in the pathogenic process of hepatitis B infection. Therefore, we evaluated the association of PvuII (rs2234693) and XbaI (rs9340799) in ESR1 and HBV infection in Guangxi Zhuang populations.
METHODS
A total of 389 subjects were divided into four groups: 112 patients with chronic hepatitis B (CHB), 65 patients with hepatitis B virus (HBV)-related liver cirrhosis (LC), 107 patients with HBV-related hepatocellular carcinoma (HCC), and 105 healthy controls. The polymerase chain reaction-restriction fragment length polymorphism strategy was used to detect ESR1 gene PvuII and XbaI polymorphisms.
RESULTS
Compared with healthy controls, binary logistic regression analyses show that the CC genotype of PvuII was associated with a significantly increased susceptibility to CHB compared with the TT genotype (OR=1.760, 95% CI 1.316-2.831; p=0.044). The PvuII CC genotype was also associated with significantly increased risk of HBV-related LC (OR=1.921, 95% CI 1.342-2.478; p=0.043). Similarly, the subjects bearing the homozygous CC genotype of PvuII polymorphism also had more than a 1.7-fold increased risk for development of HCC (OR=1.748, 95% CI 1.313-2.787; p=0.010) compared with those bearing the TT genotype. Furthermore, the AC haplotype was associated with a significantly increased risk of HCC with an OR of 1.456 (p=0.003). In contrast, there were no significant differences in the genotype and allele of XbaI polymorphisms in the ESR1 gene between the groups of patients and healthy controls. In addition, ESR1 polymorphisms were not significantly associated with susceptibility to HBV-related HCC when using CHB and LC patients as references.
CONCLUSION
We conclude that the CC genotype of PvuII in ESR1 is associated with an increased risk of CHB, HBV-related LC and HCC in Guangxi Zhuang populations.
背景与目的
现有证据表明，雌激素受体α（ESR1）与乙型肝炎感染的致病过程有关。因此，我们评估了广西壮族人群中ESR1基因的PvuII（rs2234693）和XbaI（rs9340799）多态性与HBV感染之间的关联。
方法
总共389名受试者被分为四组：112例慢性乙型肝炎（CHB）患者、65例乙型肝炎病毒（HBV）相关肝硬化（LC）患者、107例HBV相关肝细胞癌（HCC）患者和105名健康对照者。采用聚合酶链反应-限制性片段长度多态性策略检测ESR1基因PvuII和XbaI多态性。
结果
与健康对照者相比，二元逻辑回归分析显示，与TT基因型相比，PvuII的CC基因型与CHB易感性显著增加相关（OR=1.760，95%CI 1.316-2.831；p=0.044）。PvuII CC基因型还与HBV相关LC风险显著增加相关（OR=1.921，95%CI 1.342-2.478；p=0.043）。同样，与携带TT基因型的受试者相比，携带PvuII多态性纯合CC基因型的受试者发生HCC的风险增加超过1.7倍（OR=1.748，95%CI 1.313-2.787；p=0.010）。此外，AC单倍型与HCC风险显著增加相关，OR为1.456（p=0.003）。相比之下，患者组与健康对照组之间ESR1基因XbaI多态性的基因型和等位基因无显著差异。此外，以CHB和LC患者作为对照时，ESR1多态性与HBV相关HCC的易感性无显著关联。
结论
我们得出结论，ESR1基因中PvuII的CC基因型与广西壮族人群中CHB、HBV相关LC和HCC风险增加有关。
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