Arashima Y, Iguthi K, Kawabata M, Kumasaka K, Okuyama K, Kawano K
Kansenshogaku Zasshi. 1989 Feb;63(2):125-9. doi: 10.11150/kansenshogakuzasshi1970.63.125.
It has been reported that the Pasteurella multocida does not grow on the BTB agar. Therefore, this medium has been used as selective and differential medium for Pasteurella multocida. However, we have experienced that some of the Pasteurella multocida from the patient's materials grew on the BTB agar. Here, we will report on the studies of the growth of the Pasteurella multocida strain on the BTB agar. Ten strains of Pasteurella multocida from humans and animals were used as the test strains. Those were adjusted to McFarland No. 5 by the sterilized physiological saline and inoculated on the agars. We compared commercially prepared BTB agars from 3 companies and BTB agars prepared by our-self from dehydrated culture medium. Blood, Chocolate, Nutrient and MacConkey agar were also used in this study. As for the growth of the Pasteurella multocida, we checked the pH of each agar and the temperature during the cultivation. The results are as follows: 1) Pasteurella multocida was confirmed to grow on all of the BTB agar. 2) Pasteurella multocida grew most heavily at 37 degrees C and pH of 7.4 to 8.2. 3) The difference of the growth on each agar was considered to be the difference of the pH and nutritional condition of the agar.
据报道，多杀巴斯德菌不能在BTB琼脂上生长。因此，该培养基一直被用作多杀巴斯德菌的选择性和鉴别性培养基。然而，我们发现从患者样本中分离出的一些多杀巴斯德菌能在BTB琼脂上生长。在此，我们将报告多杀巴斯德菌菌株在BTB琼脂上生长情况的研究。选用了10株来自人和动物的多杀巴斯德菌作为测试菌株。用无菌生理盐水将其调整至麦氏比浊度5号，并接种于琼脂上。我们比较了3家公司商业化生产的BTB琼脂以及我们自己用脱水培养基制备的BTB琼脂。本研究还使用了血琼脂、巧克力琼脂、营养琼脂和麦康凯琼脂。关于多杀巴斯德菌的生长情况，我们检测了每种琼脂的pH值以及培养过程中的温度。结果如下：1）证实多杀巴斯德菌能在所有BTB琼脂上生长。2）多杀巴斯德菌在37℃、pH值为7.4至8.2时生长最为旺盛。3）每种琼脂上生长情况的差异被认为是琼脂pH值和营养条件的差异。
