人双阳性胸腺细胞克隆的建立。
Establishment of human double-positive thymocyte clones.
作者信息
De Libero G, Lanzavecchia A
机构信息
Basel Institute for Immunology, Switzerland.
出版信息
J Exp Med. 1989 Jul 1;170(1):303-8. doi: 10.1084/jem.170.1.303.
Abstract
Human thymocytes were sorted according to the expression of CD4 and CD8 molecules and clones representing the four subpopulations (DP, DN, and single CD4 or CD8 positive) were established. DP clones can be maintained for long periods in tissue culture and give rise to a variable percentage of SP variants. These variants, when isolated and further expanded, do not revert to a DP phenotype. DP clones express a functional TCR-CD3 complex, suggesting that this molecule can interact with the thymic microenvironment during T cell differentiation.
摘要
根据CD4和CD8分子的表达对人胸腺细胞进行分选，并建立了代表四个亚群（双阳性、双阴性以及单一CD4或CD8阳性）的克隆。双阳性克隆可在组织培养中长时间维持，并产生不同比例的单阳性变体。这些变体分离并进一步扩增后，不会恢复为双阳性表型。双阳性克隆表达功能性TCR-CD3复合物，表明该分子在T细胞分化过程中可与胸腺微环境相互作用。
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本文引用的文献
1
The differentiation and function of human T lymphocytes.人类T淋巴细胞的分化与功能。
Cell. 1980 Apr;19(4):821-7. doi: 10.1016/0092-8674(80)90072-0.
2
Regulation of T-cell receptor gene expression in human T-cell development.人类T细胞发育过程中T细胞受体基因表达的调控
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4503-7. doi: 10.1073/pnas.82.13.4503.
3
4
Functional competency of T cell antigen receptors in human thymus.人类胸腺中T细胞抗原受体的功能活性
J Immunol. 1987 Nov 15;139(10):3245-50.
5
The T cell receptor.T细胞受体。
Science. 1987 Nov 20;238(4830):1073-9. doi: 10.1126/science.3317824.
6
Deletion of self-reactive thymocytes occurs at a CD4+8+ precursor stage.自身反应性胸腺细胞的缺失发生在CD4+8+前体细胞阶段。
Nature. 1988 Aug 18;334(6183):620-3. doi: 10.1038/334620a0.
7
Tolerance in T-cell-receptor transgenic mice involves deletion of nonmature CD4+8+ thymocytes.T细胞受体转基因小鼠中的耐受性涉及未成熟CD4+8+胸腺细胞的缺失。
Nature. 1988 Jun 23;333(6175):742-6. doi: 10.1038/333742a0.
8
9
Intrathymic deletion of self-reactive cells prevented by neonatal anti-CD4 antibody treatment.
Nature. 1988 Sep 8;335(6186):174-6. doi: 10.1038/335174a0.
10
Class II major histocompatibility complex molecules regulate the development of the T4+T8- inducer phenotype of cultured human thymocytes.II类主要组织相容性复合体分子调节培养的人胸腺细胞T4+T8-诱导型表型的发育。
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