Universitat Politècnica de València, Instituto de Investigación para la Gestión Integrada de Zonas Costeras (IGIC), C/ Paranimf n°1, 46730 Grao de Gandía, Valencia, Spain.
Appl Spectrosc. 2014;68(6):642-8. doi: 10.1366/13-07330.
A new and sensitive application of chemiluminescence detection has been developed for the determination of the pesticide thiacloprid in water. It was based on the on-line photoreaction of thiacloprid in a basic medium, with quinine acting as the sensitizer of the chemiluminescent response; cerium (IV) in sulfuric acid medium was used as the oxidant. High degrees of automation and reproducibility were achieved using a flow-injection analysis (FIA) manifold. The validation of the method was performed in terms of selectivity, linearity, limit of detection (LOD), precision, and accuracy. Liquid chromatography with ultraviolet (UV) detection was used as reference for mineral, tap, ground, and spring water samples. The proposed method is fast (with a throughput of 130 h(-1)), sensitive (LOD of 0.8 ng mL(-1) without preconcentration steps and of 0.08 ng mL(-1) with solid-phase extraction [SPE]), low cost, and possible to couple with separation methods for the simultaneous determination of other pesticides. The enhanced chemiluminescence intensity was linear with the thiacloprid concentration above the 2-80 and 80-800 ng mL(-1) ranges. A possible reaction mechanism is also discussed.
一种新的、灵敏的化学发光检测方法已被开发用于水中农药噻虫啉的测定。它是基于噻虫啉在碱性介质中的在线光反应，而奎宁则作为化学发光响应的敏化剂；硫酸介质中的铈（IV）被用作氧化剂。通过使用流动注射分析（FIA）流路，实现了高度的自动化和重现性。该方法的验证是在选择性、线性、检测限（LOD）、精密度和准确性方面进行的。采用紫外（UV）检测的液相色谱法作为矿泉水、自来水、地下水和泉水样品的参考方法。该方法快速（通量为 130 h(-1)）、灵敏（无需预浓缩步骤的检测限为 0.8 ng mL(-1)，固相萃取（SPE）的检测限为 0.08 ng mL(-1)）、低成本，并且可以与分离方法结合用于同时测定其他农药。增强的化学发光强度与噻虫啉浓度呈线性关系，范围在 2-80 和 80-800 ng mL(-1)之间。还讨论了一种可能的反应机制。
