Baer G Ia, Emets A I, Blium Ia B
Tsitol Genet. 2014 May-Jun;48(3):3-11.
The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAm 1), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 microM of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgene nature of plants. The analysis of seed of T1 oftransgene lines confirmed heterozygous character of inheritance of the resistance.
当前数据致力于研究采用携带从R-生物型牛筋草（蟋蟀草）中分离出的突变α-微管蛋白基因（TUAm 1）的构建体，通过生物弹道法和农杆菌介导法转化黍稷，以解决二硝基苯胺抗性问题。研究发现，10微摩尔的氟乐灵是筛选黍稷转基因植株的最佳浓度。对转化株系进行PCR分析证实了植株的转基因特性。对转基因株系T1代种子的分析证实了抗性遗传的杂合特性。
