双链断裂修复中的聚（ADP - 核糖）聚合酶：聚焦于PARP1、PARP2和PARP3。

Poly(ADP-ribose) polymerases in double-strand break repair: focus on PARP1, PARP2 and PARP3.

作者信息

Beck Carole, Robert Isabelle, Reina-San-Martin Bernardo, Schreiber Valérie, Dantzer Françoise

机构信息

Poly(ADP-ribosyl)ation and Genome Integrity, Equipe labellisée Ligue Nationale Contre Le Cancer, Laboratoire d׳Excellence Medalis, UMR7242, Centre National de la Recherche Scientifique/Université de Strasbourg, Institut de Recherche de l׳Ecole de Biotechnologie de Strasbourg, bld. S. Brant, BP10413,67412 Illkirch, France.

Institut de Génétique et de Biologie Moléculaire et Cellulaire; Institut National de la Santé et de la Recherche Médicale, U964; Centre National de la Recherche Scientifique, UMR7104; Université de Strasbourg; Illkirch, 67400, France.

出版信息

Exp Cell Res. 2014 Nov 15;329(1):18-25. doi: 10.1016/j.yexcr.2014.07.003. Epub 2014 Jul 10.

DOI:10.1016/j.yexcr.2014.07.003

PMID:25017100

Abstract

Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members have highlighted their key contribution in the DNA damage response network, the best characterized being the role of PARP1 and PARP2 in the resolution of single-strand breaks as part of the BER/SSBR process. How PARylation contributes to the repair of double-strand breaks is less well defined but has become recently the subject of significant research in the field. The aim of this review is to provide an overview of the current knowledge concerning the role of the DNA-activated PARP1, PARP2 and PARP3 in cellular response to double-strand breaks (DSB). In addition, we outline the biological significance of these properties in response to programmed DNA lesions formed during physiological processes such as antibody repertoire assembly and diversification.

摘要

聚（ADP-核糖）化（PARylation）是一种由聚（ADP-核糖）聚合酶（PARP）催化的蛋白质翻译后修饰。最近在DNA依赖性PARP家族成员的生化和功能特性方面取得了大量进展，突出了它们在DNA损伤反应网络中的关键作用，其中最具代表性的是PARP1和PARP2在碱基切除修复/单链断裂修复（BER/SSBR）过程中对单链断裂修复的作用。PARylation如何促进双链断裂的修复尚不太明确，但最近已成为该领域大量研究的主题。本综述的目的是概述目前关于DNA激活的PARP1、PARP2和PARP3在细胞对双链断裂（DSB）反应中的作用的知识。此外，我们概述了这些特性在应对生理过程（如抗体库组装和多样化）中形成的程序性DNA损伤时的生物学意义。