Duarte Christiane Eliza Motta, Carvalho Carlos Roberto, Silva-Filho Agnaldo Lopes da
Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Taiwan J Obstet Gynecol. 2014 Jun;53(2):227-31. doi: 10.1016/j.tjog.2014.04.019.
To determine DNA ploidy in the cervical specimens of patients revealing a suspicion of cancer by image analysis performed by using a combination of commercial analysis software, conventional microscopy, and certified filters.
This study followed a prospective design. Cervical samples were obtained from 20 patients undergoing routine screening in the Gynecologic-Oncology Unit of the University Hospital of the Federal University of Minas Gerais, Brazil. Three slides were prepared for each case and the DNA content was determined by image cytometry, post Feulgen staining. DNA ploidy, as well as events exceeding 5C and 9C, was assessed according to the guidelines and algorithms prescribed for diagnostic interpretation by the European Society for Analytical Cellular Pathology.
By employing the adapted tool, identification of the lesions with euploid and aneuploid profiles was possible. Abnormal DNA content was found in 65% of the cases (13/20), with 45% (9/20) presenting nuclei with >5C content and 20% (4/20) with >9C content. In the analyses conducted in this study, the coefficient of variation with respect to DNA quantity was lower than the 5% threshold recommended by the European Society for Analytical Cellular Pathology.
Image cytometry of the cervical specimens revealed DNA aneuploidy, most probably resulting from chromosomal alterations and appearing as precancerous lesions in 65% of the cases. The adaptations implemented in this study, enabled the DNA-image cytometry to become more accessible, enhancing its extended use as an adjuvant strategy for the early screening of the cervical epithelium samples during routine analyses.
通过使用商业分析软件、传统显微镜和认证滤光片相结合的图像分析，确定疑似癌症患者宫颈标本中的DNA倍性。
本研究采用前瞻性设计。从巴西米纳斯吉拉斯联邦大学大学医院妇科肿瘤病房接受常规筛查的20名患者中获取宫颈样本。每个病例制备三张玻片，并通过图像细胞术在福尔根染色后测定DNA含量。根据欧洲分析细胞病理学学会规定的诊断解释指南和算法评估DNA倍性以及超过5C和9C的事件。
通过使用适配工具，可以识别具有整倍体和非整倍体特征的病变。65%（13/20）的病例发现DNA含量异常，其中45%（9/20）的细胞核含量>5C，20%（4/20）的细胞核含量>9C。在本研究进行的分析中，DNA量的变异系数低于欧洲分析细胞病理学学会推荐的5%阈值。
宫颈标本的图像细胞术显示DNA非整倍体，很可能是由染色体改变引起的，在65%的病例中表现为癌前病变。本研究中实施的改进使DNA图像细胞术更易于使用，增强了其作为常规分析中宫颈上皮样本早期筛查辅助策略的广泛应用。
