从硬骨鱼脑部分纯化并鉴定一种与大鼠下丘脑肽相关的新型生长激素释放因子（GRF）

Partial purification and characterization of a novel growth hormone-releasing factor (GRF) from teleost brain related to the rat hypothalamic peptide.

作者信息

Ackland J F, Wu P, Bruhn T O, Jackson I M

机构信息

Division of Endocrinology, Brown University, Rhode Island Hospital, Providence 02903.

出版信息

Peptides. 1989 Jan-Feb;10(1):15-9. doi: 10.1016/0196-9781(89)90068-5.

DOI:10.1016/0196-9781(89)90068-5

PMID:2501769

Abstract

A growth hormone-releasing factor (GRF)-like molecule has been partially purified and characterized from acid extracts of codfish (Gadhus morhua) brain using immunoaffinity and gel chromatography, followed by HPLC. This material has a mol.wt. which is similar to known mammalian forms of GRF but is immunologically and/or chromatographically distinct from previously described GRF peptides. However, it is related to rat(r) GRF(1-43) since it causes marked displacement in the rGRF RIA. Codfish GRF is a highly specific and potent hypophysiotropic factor as shown by its ability to stimulate the release of GH, but no other hormone, from rat anterior pituitary cells in vitro. These findings suggest that, phylogenetically, GRF is an ancient molecule with its biologic activity and certain immunoreactive domain(s) conserved, at least, from teleost to mammal.

摘要

利用免疫亲和和凝胶色谱法，随后进行高效液相色谱法，从鳕鱼（大西洋鳕）脑的酸性提取物中部分纯化并鉴定了一种生长激素释放因子（GRF）样分子。该物质的分子量与已知的哺乳动物形式的GRF相似，但在免疫和/或色谱方面与先前描述的GRF肽不同。然而，它与大鼠（r）GRF（1-43）相关，因为它在rGRF放射免疫分析中引起明显的置换。鳕鱼GRF是一种高度特异性和强效的促垂体激素因子，体外实验表明它能够刺激大鼠垂体前叶细胞释放生长激素，但不释放其他激素。这些发现表明，从系统发育角度来看，GRF是一种古老的分子，其生物活性和某些免疫反应结构域至少从硬骨鱼到哺乳动物都得到了保留。