Rola Jolanta G, Larska Magdalena, Grzeszuk Monika, Bocian Lukasz, Kuta Aleksandra, Polak Miroslaw P, Rola Jerzy
Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland.
Prev Vet Med. 2014 Sep 1;116(1-2):183-7. doi: 10.1016/j.prevetmed.2014.06.007. Epub 2014 Jun 23.
The aim of the study was to examine the effect of bovine viral diarrhoea virus (BVDV) infection on bulk tank milk somatic cell counts (BMSCC). Twenty nine dairy farms supplying milk to a dairy in Eastern Poland were recruited for the study. Bulk milk ELISA and RT-PCR were used to determine the BVDV infection status and the presence of PI animals in the farms. The BMSCC mean values for the BVDV seronegative (218.7 × 10(3)cells/ml; SD: 89.8) and seropositive (214.9 × 10(3)cells/ml; SD: 74.0) herds did not differ significantly. To assess the relationship between BVDV infection and BMSCC a multilevel mixed-effects linear model was used. No statistically significant effect of BVDV infection on BMSCC was found. The mean values of BMSCC for the herds with PI individuals measured before (230.1 × 10(3)cells/ml, SD: 64.9) and after (223.3 × 10(3)cells/ml, SD: 62.4) the PI removal were not statistically different. An increase in herd size was associated with a significant decrease in BMSCC. An increase in BMSCC was observed during summer (from May to September) compared to during winter (from October to April).
本研究的目的是检测牛病毒性腹泻病毒（BVDV）感染对奶罐体细胞计数（BMSCC）的影响。招募了29个向波兰东部一家乳制品厂供应牛奶的奶牛场参与该研究。采用批量牛奶ELISA和RT-PCR检测奶牛场的BVDV感染状况及持续性感染（PI）动物的存在情况。BVDV血清阴性牛群（218.7×10³个细胞/毫升；标准差：89.8）和血清阳性牛群（214.9×10³个细胞/毫升；标准差：74.0）的BMSCC平均值无显著差异。为评估BVDV感染与BMSCC之间的关系，使用了多级混合效应线性模型。未发现BVDV感染对BMSCC有统计学上的显著影响。在清除PI个体之前（230.1×10³个细胞/毫升，标准差：64.9）和之后（223.3×10³个细胞/毫升，标准差：62.4），有PI个体的牛群的BMSCC平均值无统计学差异。牛群规模的增加与BMSCC的显著降低相关。与冬季（10月至4月）相比，夏季（5月至9月）观察到BMSCC有所增加。
