γ干扰素在病毒感染时循环淋巴细胞中的表达
Expression of gamma interferon on circulating lymphocytes in viral infections.
作者信息
Caruso A, Stellini R, Scalzini A, Pollara P, Foresti I, Bonfanti C, Scura G, Gonzales R, Manca N, Turano A
机构信息
Institute of Microbiology, University of Brescia, Italy.
出版信息
J Clin Microbiol. 1989 Jun;27(6):1426-9. doi: 10.1128/jcm.27.6.1426-1429.1989.
Peripheral blood lymphocytes from a total of 111 patients and 40 healthy individuals were studied for gamma interferon (IFN-gamma) expression on their surfaces by indirect immunofluorescence assay and flow cytometry, with a new anti-IFN-gamma monoclonal antibody (IGMB-14) as a specific reagent. Of 64 patients with proven acute viral infections, 59 had a significantly higher percentage of lymphocytes expressing IFN-gamma on their membranes than healthy individuals did. On the other hand, only 3 (8.9%) of 34 patients with proven bacterial infections had an increased percentage of IFN-gamma-expressing lymphocytes. None of the eight patients with other infections and none of the five with systemic lupus erythematosus showed an increased percentage of IFN-gamma-positive lymphocytes. The percentage of IFN-gamma-expressing lymphocytes during a viral infection was found to be related to different stages of the disease. Finally, some applications of this rapid IFN-gamma assay method in viral diseases are discussed.
采用间接免疫荧光法和流式细胞术，以一种新型抗γ干扰素单克隆抗体（IGMB - 14）作为特异性试剂，研究了111例患者和40名健康个体外周血淋巴细胞表面γ干扰素（IFN - γ）的表达情况。在64例确诊为急性病毒感染的患者中，59例患者细胞膜上表达IFN - γ的淋巴细胞百分比显著高于健康个体。另一方面，在34例确诊为细菌感染的患者中，只有3例（8.9%）表达IFN - γ的淋巴细胞百分比有所增加。8例其他感染患者和5例系统性红斑狼疮患者中，均未发现IFN - γ阳性淋巴细胞百分比增加。发现病毒感染期间表达IFN - γ的淋巴细胞百分比与疾病的不同阶段有关。最后，讨论了这种快速IFN - γ检测方法在病毒疾病中的一些应用。
Zotero 插件