Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran ; Tehran University of Medical Sciences, Biotechnology Research Center, Tehran, Iran.
Tehran University of Medical Sciences, Biotechnology Research Center, Tehran, Iran.
Turk J Haematol. 2014 Jun;31(2):149-54. doi: 10.4274/tjh.2012.0164. Epub 2014 Jun 10.
One of the major goals of cancer treatment is the monitoring of chemotherapeutic protocols. Quantitative and comparative cytokine expression profiling could be reliable to be used for biomarkers in deadly and fast-growing cancers such as acute myeloid leukemia (AML). The present study aims to assess and further validate cytokines with probable effects on proliferation and maturation of blood cells in AML.
Gene expression levels of IL-1β, IL-10, IL-8, TNF-α, and IFN-γ were analyzed before and after chemotherapy and after granulocyte colony-stimulating factor (G-CSF) therapy in 46 AML patients by an in-house quantitative comparative RT-PCR method.
Our findings indicated that although the gene expression level of TNF-α was almost constant in all 3 samples, IL-1β, IL-8, and IL-10 expression levels showed a decrease after chemotherapy and an increase after G-CSF therapy. On the other hand, the expression level of IFN-γ had a different pattern with an increase after chemotherapy and a decrease after G-CSF therapy.
Taken together, the results of this study are in support of the idea that the analyzed cytokines could be useful biomarkers for AML treatment monitoring. However, further molecular epidemiological investigations are suggested to elaborate more cancer monitoring biomarkers.
癌症治疗的主要目标之一是监测化疗方案。定量和比较细胞因子表达谱可作为生物标志物，用于监测致命且快速生长的癌症，如急性髓细胞白血病（AML）。本研究旨在评估和进一步验证可能对 AML 中血细胞增殖和成熟有影响的细胞因子。
通过内部定量比较 RT-PCR 方法，分析 46 例 AML 患者化疗前后及粒细胞集落刺激因子（G-CSF）治疗后的 IL-1β、IL-10、IL-8、TNF-α 和 IFN-γ 的基因表达水平。
我们的研究结果表明，虽然 TNF-α 的基因表达水平在所有 3 个样本中几乎保持不变，但 IL-1β、IL-8 和 IL-10 的表达水平在化疗后下降，在 G-CSF 治疗后上升。另一方面，IFN-γ 的表达水平呈不同模式，在化疗后增加，在 G-CSF 治疗后减少。
综上所述，本研究结果支持分析细胞因子可作为 AML 治疗监测的有用生物标志物的观点。然而，建议进行进一步的分子流行病学研究，以阐明更多的癌症监测生物标志物。
