Kozai Yuki, Tsuzuki Satoshi, Takai Marie, Eguchi Ai, Matsumura Shigenobu, Inoue Kazuo, Fushiki Tohru
a Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
Biosci Biotechnol Biochem. 2014;78(5):839-42. doi: 10.1080/09168451.2014.891934. Epub 2014 Jun 13.
We recently obtained evidence that unsaturated long-chain fatty acids (LCFAs) (e.g. oleic acid) inhibit binding of oxidized low-density lipoproteins (oxLDLs) to CD36. In the present study, we validated this prediction by examining inhibition by unsaturated LCFAs of Alexa-fluor-labeled oxLDL binding to multiwell plates onto which a synthetic CD36 peptide is covalently immobilized via thiol-maleimide coupling.
我们最近获得的证据表明，不饱和长链脂肪酸（LCFAs）（如油酸）可抑制氧化型低密度脂蛋白（oxLDLs）与CD36的结合。在本研究中，我们通过检测不饱和LCFAs对Alexa荧光标记的oxLDL与通过硫醇-马来酰亚胺偶联共价固定有合成CD36肽的多孔板结合的抑制作用，验证了这一预测。
