Shao Qing-Song, Liu Hong-Bo, Zhao Xiao-Fang, Hu Run-Huai, Li Ming-Yan
Zhongguo Zhong Yao Za Zhi. 2014 Apr;39(8):1386-90.
The mycelium growth and sporulation in different temperature, pH value and light conditions were detected by using the crossing and haemocytometer method. The toxicity of five fungicides to Fusarium oxysporum was tested by mycelia growth method, so as to provide the reference for prevention and control against F. oxysporum. The optimum temperature and pH value of F. oxysporum to grow and spore were 28 degrees C and 6-7. Alternating light and darkness promoted growth and sporulation of bacterial colony. As for five fungicides, the EC50 of tebuconazole was 10.02 mg x L(-1), 92.50 times as much as carbendazim. The EC50 of myclobutanil and Fuxing was 91.23, 96.68 mg x L(-1), respectively. Tebuconazole showed the most tremendous inhibitory effect and control efficiency on F. oxysporum.
采用划线法和血细胞计数板法检测不同温度、pH值和光照条件下的菌丝生长和孢子形成情况。采用菌丝生长法测定了5种杀菌剂对尖孢镰刀菌的毒力,为尖孢镰刀菌的防治提供参考。尖孢镰刀菌生长和产孢的最适温度和pH值分别为28℃和6 - 7。明暗交替促进菌落生长和产孢。对于5种杀菌剂,戊唑醇的EC50为10.02 mg·L-1,是多菌灵的92.50倍。腈菌唑和福星的EC50分别为91.23、96.68 mg·L-1。戊唑醇对尖孢镰刀菌的抑制作用和防治效果最为显著。