Manzo-Valencia María Karina, Valdés-Santiago Laura, Sánchez-Segura Lino, Guzmán-de-Peña Dora Linda
Departamento de Biotecnología y Bioquímica and ‡Departamento de Ingeniería Genética, Unidad Irapuato Centro de Investigación y Estudios Avanzados-IPN , Km 9.6 Libramiento Norte Irapuato-León, 36821, Irapuato Guanajuato, México.
J Agric Food Chem. 2016 Nov 9;64(44):8315-8323. doi: 10.1021/acs.jafc.6b03105. Epub 2016 Nov 1.
The response to exogenous addition of naphthalene acetic acid potassium salt (NAA-K) to Fusarium oxysporum f. sp radici-lycopersici ATCC 60095 and F. oxysporum f. sp. cubense isolated from Michoacan Mexico soil is reported. The in vitro study showed that NAA-K might be effective in the control of Fusarium oxysporum. Exogenous application of NAA-K affected both spores and mycelium stages of the fungi. Viability testing using acridine orange and propidium iodide showed that NAA-K possesses fungal killing properties, doing it effectively in the destruction of conidia of this phytopathogenic fungi. Analysis of treated spores by scanning electron microscopy showed changes in the shape factor and fractal dimension. Moreover, NAA-K repressed the expression of brlA and fluG genes. The results disclosed here give evidence of the use of this synthetic growth factor as a substance of biocontrol that presents advantages, and the methods of application in situ should be explored.
报道了向尖孢镰刀菌番茄根腐专化型ATCC 60095和从墨西哥米却肯州土壤中分离出的尖孢镰刀菌香蕉枯萎专化型外源添加萘乙酸钾盐（NAA-K）后的反应。体外研究表明，NAA-K可能对尖孢镰刀菌具有防治效果。NAA-K的外源施用影响了真菌的孢子和菌丝体阶段。使用吖啶橙和碘化丙啶进行的活力测试表明，NAA-K具有真菌杀伤特性，能有效破坏这种植物病原真菌的分生孢子。通过扫描电子显微镜对处理过的孢子进行分析，结果显示形状因子和分形维数发生了变化。此外，NAA-K抑制了brlA和fluG基因的表达。此处披露的结果证明了这种合成生长因子作为一种具有优势的生物防治物质的用途，应探索其原位应用方法。
