Cafaro T A, Suarez M F, Maldonado C, Croxatto J O, Insfrán C, Urrets-Zavalía J A, Serra H M
Faculty of Chemical Science, National University of Córdoba, Medina Allende, Córdoba, Argentina.
Department of Clinical Biochemistry, CIBICI, Faculty of Chemical Science, National University of Córdoba, Medina Allende, Córdoba, Argentina.
Anat Histol Embryol. 2015 Aug;44(4):247-54. doi: 10.1111/ahe.12131. Epub 2014 Jul 17.
Our study performed qualitative and quantitative studies on the corneal ultrastructure of healthy female Merino sheep of ages 4 months and 6 years old from the Argentinean Pampa. The corneas were evaluated using ex vivo laser-scanning confocal microscopy, light microscopy and transmission electron microscopy. Those studies allowed us to obtain detailed images of the corneal layers as well as quantitative data of the cellular and sub-basal nerve densities in the cornea from sheep of different ages. The density of the corneal cells was significantly different in the anterior versus the posterior epithelium and stroma. Moreover, the density of the epithelial, stromal cells and endothelial cells, as well as the sub-basal nerve density were significantly lower in adult than in young animals. Our work provided a wide-ranging description of the corneal ultrastructure of healthy female Merino sheep, which adds to the current knowledge about the ophthalmological aspects of this species and undoubtedly benefits veterinarians.
我们的研究对来自阿根廷潘帕斯草原的4个月龄和6岁健康雌性美利奴绵羊的角膜超微结构进行了定性和定量研究。使用离体激光扫描共聚焦显微镜、光学显微镜和透射电子显微镜对角膜进行评估。这些研究使我们能够获得不同年龄绵羊角膜各层的详细图像以及角膜中细胞和基底膜下神经密度的定量数据。角膜细胞密度在前部与后部上皮及基质中存在显著差异。此外，成年动物的上皮细胞、基质细胞和内皮细胞密度以及基底膜下神经密度均显著低于幼年动物。我们的工作对健康雌性美利奴绵羊的角膜超微结构进行了广泛描述，这增加了关于该物种眼科方面的现有知识，无疑对兽医有益。
