Tian Miao, Zeng Xiang-Qing, Song Huan-Lei, Hu Shan-Xin, Wang Fu-Jun, Zhao Jian, Hu Zhi-Bi
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
J Sci Food Agric. 2015 Apr;95(6):1328-35. doi: 10.1002/jsfa.6826. Epub 2014 Aug 4.
Momordica charantia (MC) has been used for treating diabetes mellitus from ancient times in Asia, Africa and South America. There are many MC accessions in local markets. Polypeptide-P as a main hypoglycemic component in MC was first studied in this experiment to illustrate the different contents in MC of different accessions and different harvesting times.
Nineteen MC accessions collected from different regions were clustered into three groups using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular markers. Content of polypeptide-P in the tested MC accessions was detected by western blot (WB) method. The WB results revealed that polypeptide-P was detected in MC accessions harvested in June and July but not in September and October. Furthermore, Polypeptide-P content corresponded well with the MC accessions.
Our results suggest that the MC accessions and the harvesting times or the weather during harvest play significant roles in high content of polypeptide-P.
在亚洲、非洲和南美洲，苦瓜自古以来就被用于治疗糖尿病。当地市场上有许多苦瓜品种。本实验首次对苦瓜中主要的降血糖成分多肽 -P进行研究，以阐明不同品种和不同采收时间的苦瓜中多肽 -P的含量差异。
利用随机扩增多态性DNA（RAPD）和简单序列重复区间（ISSR）分子标记，将从不同地区收集的19个苦瓜品种聚类为三组。采用蛋白质免疫印迹（WB）法检测受试苦瓜品种中多肽 -P的含量。WB结果显示，6月和7月采收的苦瓜品种中检测到多肽 -P，而9月和10月采收的苦瓜品种中未检测到。此外，多肽 -P含量与苦瓜品种密切相关。
我们的结果表明，苦瓜品种以及采收时间或采收时的天气对多肽 -P的高含量起着重要作用。
