Debey P, Renard J P, Geze M, Adenot P
Unité en Développement Concerté I.N.S.E.R.M.-I.N.R.A., Institut de Biologie Physico-Chimique, Paris.
C R Acad Sci III. 1989;309(6):197-202.
Video enhanced fluorescence microscopy coupled to digitized image processing was used to follow the dynamics of chromatin changes in live one cell mouse embryos. The experimentation on live material was made possible through the use of very low concentrations of the DNA specific fluorophore, Hoechst 33342, and very low irradiation intensities, which maintain a good viability of embryos.
结合数字化图像处理的视频增强荧光显微镜被用于追踪活的单细胞小鼠胚胎中染色质变化的动态过程。通过使用极低浓度的DNA特异性荧光团Hoechst 33342和极低的照射强度，使得对活材料进行实验成为可能，这些措施保持了胚胎良好的活力。
