Shih Mei Fen, Cherng Jong Yuh
Department of Pharmacy, Chia-Nan University of Pharmacy & Science, Tainan 717, Taiwan.
Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi 621, Taiwan.
Molecules. 2014 Jul 18;19(7):10534-45. doi: 10.3390/molecules190710534.
The inflammatory reaction in large blood vessels involves up-regulation of vascular adhesion molecules such as endothelial cell selectin (E-selectin), soluble vascular cell adhesion molecule (sVCAM)-1, and soluble intercellular adhesion molecule (sICAM)-1. These vascular dysfunctions are associated with the development of atherosclerosis. β-Amyrin, an active component of Euphorbia hirta L., has potent anti-inflammatory effects. So far, its preventive effects against the expression of inflammatory mediator-induced adhesion molecules have not been investigated. Endothelial cells (SVEC4-10 cell line) were treated with 50% RAW conditioned media (i.e., normal SVEC4-10 culture media contains 50% of lipopolysaccharide-activated macrophage culture media) without or with β-amyrin (0.6 and 0.3 µM). The production levels of E-selectin, sICAM-1, and sVCAM-1 in the SVEC4-10 cells were measured with ELISA assay kits. Under the same treatment conditions, expression of endothelin (ET)-1 and endothelial type of NO synthase (eNOS) mRNA were analyzed by RT-PCR and agarose gel. With β-amyrin, the 50% RAW conditioned media-induced E-selectin, sICAM-1, and sVCAM-1 levels as well as ET-1 gene expression were all suppressed. β-Amyrin treatment also restored the 50% RAW conditioned media-suppressed eNOS mRNA expression. These data indicate that β-amyrin is potentially useful in preventing chronic inflammation-related vascular diseases.
大血管中的炎症反应涉及血管黏附分子的上调，如内皮细胞选择素（E-选择素）、可溶性血管细胞黏附分子（sVCAM）-1和可溶性细胞间黏附分子（sICAM）-1。这些血管功能障碍与动脉粥样硬化的发展有关。β-香树脂醇是大戟的一种活性成分，具有强大的抗炎作用。到目前为止，尚未研究其对炎症介质诱导的黏附分子表达的预防作用。用50%RAW条件培养基（即正常的SVEC4-10培养基含有50%脂多糖激活的巨噬细胞培养基）处理内皮细胞（SVEC4-10细胞系），添加或不添加β-香树脂醇（0.6和0.3µM）。用ELISA试剂盒检测SVEC4-10细胞中E-选择素、sICAM-1和sVCAM-1的产生水平。在相同处理条件下，通过RT-PCR和琼脂糖凝胶分析内皮素（ET）-1和内皮型一氧化氮合酶（eNOS）mRNA的表达。使用β-香树脂醇时，50%RAW条件培养基诱导的E-选择素、sICAM-1和sVCAM-1水平以及ET-1基因表达均受到抑制。β-香树脂醇处理还恢复了50%RAW条件培养基抑制的eNOS mRNA表达。这些数据表明，β-香树脂醇在预防慢性炎症相关血管疾病方面可能具有潜在用途。
