使用生物素化探针检测甲状腺滤泡旁细胞中的降钙素和降钙素基因相关肽mRNA。
Detection of calcitonin and CGRP mRNAs in thyroid parafollicular cells using biotinylated probes.
作者信息
Zabel M, Schäfer H
机构信息
Department of Histology, Medical Academy, Poznań, Poland.
出版信息
Acta Histochem Suppl. 1989;37:117-21.
PMID:2505306
Abstract
Probes for hybridocytochemistry were produced using SP6 RNA polymerase system and biotinylated 11-UTP as a label. Probes were obtained capable of detecting calcitonin-mRNA and CGRP-mRNA. Probe quality was tested using Southern blotting. Hybridocytochemistry on rat thyroid sections demonstrated both mRNAs in all parafollicular cells.
摘要
使用SP6 RNA聚合酶系统和生物素化的11-UTP作为标记物制备用于杂交细胞化学的探针。获得了能够检测降钙素mRNA和降钙素基因相关肽mRNA的探针。使用Southern印迹法检测探针质量。对大鼠甲状腺切片进行的杂交细胞化学显示，所有滤泡旁细胞中均存在这两种mRNA。
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