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用杂交细胞化学法对大鼠甲状腺滤泡旁细胞中降钙素及降钙素基因相关肽mRNA进行定位。

Localization of calcitonin and calcitonin gene-related peptide mRNAs in rat parafollicular cells by hybridocytochemistry.

作者信息

Zabel M, Schäfer H

机构信息

Institut of Pathology, University of Hamburg, Federal Republic of Germany.

出版信息

J Histochem Cytochem. 1988 May;36(5):543-6. doi: 10.1177/36.5.3258608.

DOI:10.1177/36.5.3258608
PMID:3258608
Abstract

By use of appropriate fragments of CT DNA or a CGRP DNA and SP6 polymerase system, we produced anti-sense RNA probes labeled with biotinylated 11-UTP. The labeling and specificity of the RNA probes were confirmed using dot-blot hybridization. By use of hybridocytochemistry, CT mRNA and CGRP mRNA were localized in all parafollicular cells in control and dihydrotachysterin-pre-treated rats. We concluded that all parafollicular cells simultaneously produce both CT mRNA and CGRP mRNA, either under control conditions or after stimulation by dihydrotachysterin-induced hypercalcemia.

摘要

通过使用CT DNA或降钙素基因相关肽（CGRP）DNA的合适片段以及SP6聚合酶系统，我们制备了用生物素化的11-UTP标记的反义RNA探针。使用斑点印迹杂交法确认了RNA探针的标记和特异性。通过杂交细胞化学方法，在对照大鼠和二氢速甾醇预处理的大鼠中，降钙素（CT）mRNA和降钙素基因相关肽（CGRP）mRNA定位于所有滤泡旁细胞中。我们得出结论，无论是在对照条件下还是在二氢速甾醇诱导的高钙血症刺激后，所有滤泡旁细胞都同时产生CT mRNA和CGRP mRNA。

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Localization of calcitonin and calcitonin gene-related peptide mRNAs in rat parafollicular cells by hybridocytochemistry.用杂交细胞化学法对大鼠甲状腺滤泡旁细胞中降钙素及降钙素基因相关肽mRNA进行定位。
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2
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引用本文的文献

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Effect of follicular cells on calcitonin gene expression in thyroid parafollicular cells in cell culture.细胞培养中滤泡细胞对甲状腺滤泡旁细胞降钙素基因表达的影响。
Histochem J. 1999 Mar;31(3):175-80. doi: 10.1023/a:1003597416140.
2
Hybridocytochemical and immuno-ultrastructural study of calcitonin gene expression in cultured medullary carcinoma cells.培养的髓样癌细胞中降钙素基因表达的杂交细胞化学和免疫超微结构研究。
Histochemistry. 1994 Oct;102(4):323-7. doi: 10.1007/BF00269170.
3
Detection of primary and mature transcripts of calcitonin-gene-related peptide genes in rat parafollicular cells by light, fluorescence and confocal microscopy.
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Histochem Cell Biol. 1995 Jan;103(1):19-24. doi: 10.1007/BF01464471.
4
Effect of hypercalcemia on parafollicular cells in the rat thyroid gland.高钙血症对大鼠甲状腺滤泡旁细胞的影响。
Histochemistry. 1988;88(3-6):623-8. doi: 10.1007/BF00570333.
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Non-isotopic RNA probes. Comparison between different labels and detection systems.非同位素RNA探针。不同标记物与检测系统之间的比较。
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6
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