活体眼线虫（彼得罗维氧旋尾线虫）提取、体外培养及用于实验研究的转移

Live eyeworm (Oxyspirura petrowi) extraction, in vitro culture, and transfer for experimental studies.

作者信息

Dunham N R, Soliz L A, Brightman A, Rollins D, Fedynich A M, Kendall R J

机构信息

The Institute of Environmental and Human Health, Texas Tech University, Box 41163, Lubbock, Texas 79409-1163;

出版信息

J Parasitol. 2015 Feb;101(1):98-101. doi: 10.1645/13-448.1. Epub 2014 Jul 24.

DOI:10.1645/13-448.1

PMID:25059291

Abstract

Northern bobwhite ( Colinus virginianus ) have experienced a dramatic decline in West Texas over the last 3 yr, and investigations are underway to evaluate the role of parasites in this decline. One of the key parasites being investigated is the eyeworm (Oxyspirura petrowi). Live eyeworms were extracted from both live and dead northern bobwhites, and in vitro survival was tested using 10 liquid media. Eyeworms placed in an egg white and physiological saline solution lived for at least 36 days. Live O. petrowi placed into the eyes of uninfected pen-raised bobwhites were monitored for 21 days to demonstrate successful transfer. Eyeworm behavior during feeding, mating, and development were monitored. This study is important to research that requires "banking" of live O. petrowi from wild-captured definitive hosts for life history studies and assessing the impact of O. petrowi on host individuals.

摘要

在过去三年里，德克萨斯州西部的北部 bobwhite（Colinus virginianus）数量急剧下降，目前正在进行调查以评估寄生虫在这种下降中所起的作用。正在调查的关键寄生虫之一是眼线虫（Oxyspirura petrowi）。从活的和死的北部 bobwhite 中提取活的眼线虫，并使用 10 种液体培养基测试其体外存活情况。置于蛋清和生理盐溶液中的眼线虫存活了至少 36 天。将活的 O. petrowi 放入未感染的圈养 bobwhite 的眼睛中，监测 21 天以证明成功转移。监测了眼线虫在进食、交配和发育过程中的行为。这项研究对于需要从野生捕获的终末宿主“储存”活的 O. petrowi 以进行生活史研究和评估 O. petrowi 对宿主个体影响的研究很重要。