Becker Andrea C, Dengjel Jörn
Department of Dermatology, Medical Center - University of Freiburg, Hauptstr. 7, 79104, Freiburg, Germany.
Methods Mol Biol. 2014;1188:271-9. doi: 10.1007/978-1-4939-1142-4_19.
Autophagy is one of the two major degradation pathways within eukaryotic cells. Nevertheless, little is known about the protein composition of autophagosomes, the vesicles shuttling proteins to lysosomes for degradation. Protein correlation profiling in combination with stable isotope labeling by amino acids in cell culture is a stringent method to investigate the dynamics of the autophagosomal proteome. It enables the discrimination between autophagosomal and co-purifying proteins identifying organellar candidate proteins for further investigation.
自噬是真核细胞内两种主要的降解途径之一。然而，人们对自噬体（将蛋白质运输到溶酶体进行降解的囊泡）的蛋白质组成知之甚少。蛋白质关联谱分析结合细胞培养中氨基酸的稳定同位素标记是一种严格的方法，用于研究自噬体蛋白质组的动态变化。它能够区分自噬体蛋白和共纯化蛋白，从而识别出用于进一步研究的细胞器候选蛋白。
