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科威特骆驼的布鲁氏菌病

Brucellosis of camels in Kuwait.

作者信息

Al-Khalaf S, el-Khaladi A

机构信息

Agriculture Affairs and Fish Resources Authority, Safat, Kuwait.

出版信息

Comp Immunol Microbiol Infect Dis. 1989;12(1-2):1-4. doi: 10.1016/0147-9571(89)90002-7.

Abstract

This study investigated the presence of Brucella antibodies in serum and milk obtained from camels in Kuwait. Brucella strains were also isolated from the foetus using standard technique (Webridge Lab Techniques). Three serological tests for serum were adopted. These tests were Rose Bengal Plate Test (RBPT), the Serum Tube Agglutination Test (SAT) and the Complement Fixation Test (CFT). The RBPT was used for all sera samples, and both SAT and CET were used for the positive RBPT. Camels that showed a titer of 1:40 in SAT or 1:5 in CFT or greater were considered positive. Thirteen of the samples examined were found positive by CFT (at 1:5); by SAT, they showed a titer of 1:20. One serological test, the Milk Ring Test (MRT), was used for milk. Here 3 and 2 were considered positive reactors but 1+ was considered suspicious. We were unable to isolate the Brucella organism from Sedemine and Cream of milk, but we isolated them from Foetus Brucella abortus and it is confirmed by Webridge Laboratory, U.K. It is Brucella abortus (Biovar 1). The prevalence rate was 14.8% from serum by the CFT and RBPT methods and 10.8% by the SAT method. For milk, the prevalence rate was 8.0%. Two Brucella abortus were isolated from 5 foetuses.

摘要

本研究调查了科威特骆驼血清和乳汁中布鲁氏菌抗体的存在情况。还采用标准技术(韦布里奇实验室技术)从胎儿中分离出布鲁氏菌菌株。对血清采用了三种血清学检测方法。这些检测方法分别是虎红平板凝集试验(RBPT)、试管凝集试验(SAT)和补体结合试验(CFT)。所有血清样本均采用RBPT检测,SAT和CET则用于RBPT检测呈阳性的样本。SAT效价达到1:40或CFT效价达到1:5及以上的骆驼被视为阳性。通过CFT检测(效价为1:5),13个检测样本呈阳性;通过SAT检测,其效价为1:20。对乳汁采用了一种血清学检测方法,即乳环状试验(MRT)。在此试验中,3+和2+被视为阳性反应,而1+被视为可疑。我们未能从乳汁的乳清和乳脂中分离出布鲁氏菌,但从流产胎儿布鲁氏菌中分离出了该菌,并得到了英国韦布里奇实验室的确认。分离出的是流产布鲁氏菌(生物变种1)。通过CFT和RBPT方法检测血清的患病率为14.8%,通过SAT方法检测的患病率为10.8%。乳汁的患病率为8.0%。从5个胎儿中分离出了两株流产布鲁氏菌。

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