Fujimori K, Nakamura R M, Tonetta S A, Tsutsumi I, diZerega G S
Livingston Reproductive Biology Laboratory, Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles.
Endocrinol Jpn. 1989 Apr;36(2):175-85. doi: 10.1507/endocrj1954.36.175.
In order to investigate the action point of intraphysiological or supraphysiological elevation of FSH during the preovulatory period on follicular development, adult guinea pigs underwent unilateral ovariectomy on days 10, 12 and 14 of the estrous cycle (N = 6 each group). Thereafter, guinea pigs were injected twice daily with either vehicle or pregnant mare's serum gonadotropin (PMS). After 2 days, the remaining ovaries were removed. The resected ovaries were fixed, embedded in paraffin, serially sectioned (7 microns) and stained with Azan. All follicles greater than 70 microns were classified by size and atretic stage. The follicular size distribution was not affected by hemicastration at day 10, although the ratio of atretic follicles (greater than 400 microns) decreased from 51% to 32% (P less than 0.01). Hemicastration at day 12 increased the largest nonatretic population (70-99 microns group) from 17% to 26%, and the ratio of atretic follicles (greater than 400 microns) decreased from 35% to 23%. The peak size distribution of follicles was shifted from 70-99 microns to 200-299 microns by PMS, and follicles 600-899 microns in size contained an increased percentage of atresia, which resulted in the bimodal distribution of viable follicles greater than 400 microns. These data suggest that 2 day hemicastration promotes an influx of primordial follicles into growing follicles and suppresses the atretic process by a different mechanism depending on the date of hemicastration in the estrous cycle. Conversely, hemicastration + PMS accelerated viable follicle growth to increase the percentage of atresia.
为了研究排卵前期促卵泡激素（FSH）的生理或超生理升高对卵泡发育的作用点，成年豚鼠在发情周期的第10、12和14天接受单侧卵巢切除术（每组N = 6）。此后，豚鼠每天注射两次溶剂或孕马血清促性腺激素（PMS）。2天后，切除剩余的卵巢。将切除的卵巢固定、石蜡包埋、连续切片（7微米）并进行偶氮胭脂染色。所有大于70微米的卵泡按大小和闭锁阶段分类。尽管闭锁卵泡（大于400微米）的比例从51%降至32%（P < 0.01），但第10天的半侧卵巢切除对卵泡大小分布没有影响。第12天的半侧卵巢切除使最大的非闭锁卵泡群体（70 - 99微米组）从17%增加到26%，闭锁卵泡（大于400微米）的比例从35%降至23%。PMS使卵泡的峰值大小分布从70 - 99微米转移到200 - 299微米，600 - 899微米大小的卵泡闭锁百分比增加，导致大于400微米的存活卵泡出现双峰分布。这些数据表明，2天的半侧卵巢切除促进原始卵泡流入生长卵泡，并根据发情周期中半侧卵巢切除的日期通过不同机制抑制闭锁过程。相反，半侧卵巢切除 + PMS加速了存活卵泡的生长，增加了闭锁百分比。
