采用气相色谱-质谱联用法定量测定人血浆中苯二氮䓬类拮抗剂氟马西尼。

Quantitation of the benzodiazepine antagonist flumazenil in human plasma by gas chromatography-mass spectrometry.

作者信息

Fukuda E K, Choma N, Davis P P

机构信息

Department of Drug Metabolism, Hoffmann-La Roche Inc., Nutley, NJ 07110.

出版信息

J Chromatogr. 1989 Jun 30;491(1):97-106. doi: 10.1016/s0378-4347(00)82823-6.

DOI:10.1016/s0378-4347(00)82823-6

PMID:2507567

Abstract

A gas chromatographic-mass spectrometric procedure has been developed for the quantitation of the benzodiazepine antagonist flumazenil in human plasma. The assay utilizes an extraction at alkaline pH with benzene-dichloroethane (80:20), selective ion monitoring, isobutane positive-ion chemical ionization mass spectrometry and stable isotope dilution. The method has been used to measure plasma concentrations of flumazenil in over 1500 clinical samples over a range of 0.5-200 ng/ml (using 2 ml of plasma).

摘要

已开发出一种气相色谱 - 质谱分析法，用于定量测定人血浆中苯二氮䓬拮抗剂氟马西尼。该测定法采用在碱性pH值下用苯 - 二氯乙烷（80:20）进行萃取、选择性离子监测、异丁烷正离子化学电离质谱分析以及稳定同位素稀释。该方法已用于测定1500多个临床样本中氟马西尼的血浆浓度，浓度范围为0.5 - 200 ng/ml（使用2 ml血浆）。