Martin-Sampedro Raquel, Miranda Jesús, García-Fuentevilla Luisa L, Hernández Manuel, Arias Maria E, Diaz Manuel J, Eugenio Maria E
Cellulose and Paper Laboratory, Forest Product Department, INIA-CIFOR, Carretera de la Coruña, km 7.5, 28040, Madrid, Spain,
Bioprocess Biosyst Eng. 2015 Jan;38(1):113-23. doi: 10.1007/s00449-014-1249-7. Epub 2014 Aug 2.
A laccase stage can be used as a pre-treatment of a standard chemical bleaching sequence to reduce environmental concerns associated to this process. The importance of each independent variable and its influence on the properties of the bleached pulp have been studied in depth in this work, using an adaptive network-based fuzzy inference system (ANFIS) with four independent variables (laccase, buffer, mediator and oxygen) as input. Eucalyptus globulus kraft pulp was biobleached using a laccase from Pycnoporus sanguineus and a natural mediator (acetosyringone). Later, an alkaline extraction and a hydrogen peroxide treatment were applied. Most biobleaching processes showed a decrease in kappa number and an increase in brightness with no significant impact on the viscosity values, compared with the control. Oxygen was the variable with the smallest influence on the final pulp properties while the laccase and buffer solution showed a significant influence.
漆酶阶段可作为标准化学漂白序列的预处理，以减少与该过程相关的环境问题。在这项工作中，使用基于自适应网络的模糊推理系统（ANFIS），以四个自变量（漆酶、缓冲剂、介质和氧气）为输入，深入研究了每个自变量的重要性及其对漂白浆性能的影响。使用血红密孔菌的漆酶和天然介质（乙酰丁香酮）对桉木硫酸盐浆进行生物漂白。随后，进行碱萃取和过氧化氢处理。与对照相比，大多数生物漂白过程显示卡伯值降低，亮度增加，而对粘度值没有显著影响。氧气是对最终纸浆性能影响最小的变量，而漆酶和缓冲溶液显示出显著影响。
