Hill Joseph, Lawrence Jessica, Saba Corey, Turek Michelle, Feldhaeusser Brittany, Coutermarsh-Ott Sheryl, Barber Jamie, Smith Jo, Gogal Robert
Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA.
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA; Veterinary Cancer Centre, The Royal School of Veterinary Studies, University of Edinburgh, UK.
Res Vet Sci. 2014 Oct;97(2):348-56. doi: 10.1016/j.rvsc.2014.07.006. Epub 2014 Jul 15.
Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle.
猫注射部位肉瘤（ISS）是一种局部侵袭性肿瘤，在治疗中手术治疗常与放疗或化疗联合使用以提高肿瘤控制效果。本研究的重点是评估阿霉素或依托泊苷对猫注射部位肉瘤细胞系（JB）的细胞毒性作用，并评估联合使用这些药物对细胞死亡和细胞周期的影响。单药及联合用药均增加了细胞死亡并显著减少了活细胞数量。处理后，G0/G1期细胞显著减少，而G2/M期细胞比例显著增加。总体而言，以较低的半数有效浓度（EC）联合使用阿霉素和依托泊苷，在细胞毒性程度、凋亡水平以及G2/M期细胞百分比方面，产生了与单独使用任一药物的半数有效浓度（EC50）相当的结果。本研究结果表明，阿霉素和依托泊苷单独使用及联合使用对ISS细胞活力和周期有不同影响。
