从羊奶酪中分离和鉴定双歧杆菌。

Isolation and characterization of bifidobacteria from ovine cheese.

作者信息

Bunesova Vera, Killer Jiri, Vlkova Eva, Musilova Sarka, Tomaska Martin, Rada Vojtech, Kmet Vladimir

机构信息

Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 165 21, Czech Republic; Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4-Krč 14 200, Czech Republic.

出版信息

Int J Food Microbiol. 2014 Oct 1;188:26-30. doi: 10.1016/j.ijfoodmicro.2014.07.001. Epub 2014 Jul 22.

DOI:10.1016/j.ijfoodmicro.2014.07.001

PMID:25086349

Abstract

Animal products are one of the niches of bifidobacteria, a fact probably attributable to secondary contamination. In this study, 2 species of the genus Bifidobacterium were isolated by culture-dependent methods from ovine cheeses that were made from unpasteurized milk without addition of starter cultures. The isolates were identified as Bifidobacterium crudilactis and Bifidobacterium animalis subsp. lactis using matrix-assisted laser desorption/ionization time-of-flight analysis and sequencing of phylogenetic markers (16S rRNA, hsp60, and fusA).

摘要

动物制品是双歧杆菌的生态位之一，这一事实可能归因于二次污染。在本研究中，通过依赖培养的方法从由未杀菌牛奶制成且未添加发酵剂的绵羊奶酪中分离出2种双歧杆菌属菌株。使用基质辅助激光解吸/电离飞行时间分析和系统发育标记物（16S rRNA、hsp60和fusA）测序，将分离菌株鉴定为粗糙双歧杆菌和动物双歧杆菌乳酸亚种。